Human F2RL1 Stable Cell Line-HeLa

Name: Human F2RL1 Stable Cell Line-HeLa
SKU: CSC-RG1745
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1745

Host Cell : HeLa Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1745
Background	Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor.
Target Gene	F2RL1
Gene Species	Homo sapiens (Human)
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Source	HeLa
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	F2RL1 coagulation factor II (thrombin) receptor-like 1 [ Homo sapiens ]
Gene Symbol	F2RL1
Synonyms	PAR2; GPR11
Gene ID	2150
Uni Prot ID	P55085
m RNA Refseq	BC018130
Chromosome Location	5q13
Function	G-protein alpha-subunit binding;G-protein beta-subunit binding;G-protein coupled receptor activity;G-protein coupled receptor activity;protein binding;
Pathway	African trypanosomiasis, organism-specific biosystem;African trypanosomiasis, conserved biosystem;Class A/1 (Rhodopsin-like receptors), organism-specific biosystem;G alpha (q) signalling events, organism-specific biosystem;GPCR downstream signaling, organism-specific biosystem;GPCR ligand binding, organism-specific biosystem;GPCRs, Class A Rhodopsin-like, organism-specific biosystem;
MIM	600933

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