Human CRHR2-tGFP Stable Cell Line-CHO-K1

Name: Human CRHR2-tGFP Stable Cell Line-CHO-K1
SKU: CSC-RG0029
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0029

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0029
Background	Corticotropin releasing hormone receptor 2 is the gene encodes a protein that is one of two Corticotropin releasing hormone receptors (CRHRs), also termed as CRFRs. The Corticotropin releasing hormone receptor family is a group of G-coupled receptors whose principal ligand is the corticocotropin releasing hormone (CRHR or CRF). CRH is a 41-amino acid peptide synthesized in the hypothalamus that is the principal neuroregulator of the hypothalamicpituitary-adrenocortical axis and plays an important role in coordinating the endocrine, autonomic, and behavioural responses to stress and immune challenge.
Target Gene	CRHR2
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCRHR2-tGFP
Alias	CRHR2, CRF RB, CRF2, CRFR2, CRF-RB
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CRHR2 corticotropin releasing hormone receptor 2 [ Homo sapiens ]
Gene Symbol	CRHR2
Synonyms	CRF2; CRFR2; CRF-RB; HM-CRF
GeneID	12922
Uni ProtID	Q13324
mRNA Refseq	NM_001202475.1
Protein Refseq	NP_001189404.1
Chromosome Location	7p14.3
Function	corticotrophin-releasing factor receptor activity; hormone activity; protein binding;
Pathway	Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class B Secretin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	602034

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Customer Reviews

Customer Q&As

Are there any specific protocols or conditions required for culturing and maintaining the Human CRHR2-tGFP Stable Cell Line-CHO?

A: Yes, maintaining the Human CRHR2-tGFP Stable Cell Line-CHO involves specific culture conditions, such as using appropriate culture media, maintaining proper pH, and ensuring proper temperature and CO2 levels. Detailed protocols for cell culture and maintenance are typically provided by the supplier or research institution.

What are some common downstream applications or assays that researchers perform using the Human CRHR2-tGFP Stable Cell Line-CHO?

A: Researchers commonly use the Human CRHR2-tGFP Stable Cell Line-CHO in various assays, including ligand binding studies, functional assays to measure intracellular calcium release, and monitoring receptor internalization in response to ligand stimulation. It is also useful for high-throughput screening of potential CRHR2-targeting drugs.

Could you provide insights into the potential implications of studying CRHR2 using the Human CRHR2-tGFP Stable Cell Line-CHO in the context of stress-related disorders or diseases?

A: Investigating CRHR2 in the Human CRHR2-tGFP Stable Cell Line-CHO can contribute to our understanding of its role in stress-related disorders and diseases like anxiety, depression, and post-traumatic stress disorder. This knowledge can lead to the development of targeted therapies or interventions.

How stable is the expression of tGFP in the Human CRHR2-tGFP Stable Cell Line-CHO over extended culture periods, and are there any factors that can affect its stability?

A: The stability of tGFP expression in the Human CRHR2-tGFP Stable Cell Line-CHO can vary depending on various factors, including the passage number, culture conditions, and cell handling techniques. It is crucial to monitor and maintain the cells properly to ensure consistent tGFP expression.

Are there any limitations or challenges associated with using the Human CRHR2-tGFP Stable Cell Line-CHO in research, and how can these challenges be mitigated?

A: Some limitations may include the potential for genetic drift in stable cell lines and the need for consistent maintenance. To mitigate these challenges, regular monitoring, cryopreservation of early passages, and stringent quality control are recommended.

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