Cat.No. : CSC-RG0029 Host Cell: CHO-K1
| Cat. No. | CSC-RG0029 |
| Background | Corticotropin releasing hormone receptor 2 is the gene encodes a protein that is one of two Corticotropin releasing hormone receptors (CRHRs), also termed as CRFRs. The Corticotropin releasing hormone receptor family is a group of G-coupled receptors whose principal ligand is the corticocotropin releasing hormone (CRHR or CRF). CRH is a 41-amino acid peptide synthesized in the hypothalamus that is the principal neuroregulator of the hypothalamicpituitary-adrenocortical axis and plays an important role in coordinating the endocrine, autonomic, and behavioural responses to stress and immune challenge. |
| Gene | CRHR2 |
| Gene Species | Homo sapiens (Human) |
| Alias | CRHR2, CRF RB, CRF2, CRFR2, CRF-RB |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Yes, maintaining the Human CRHR2-tGFP Stable Cell Line-CHO involves specific culture conditions, such as using appropriate culture media, maintaining proper pH, and ensuring proper temperature and CO2 levels. Detailed protocols for cell culture and maintenance are typically provided by the supplier or research institution.
A: Researchers commonly use the Human CRHR2-tGFP Stable Cell Line-CHO in various assays, including ligand binding studies, functional assays to measure intracellular calcium release, and monitoring receptor internalization in response to ligand stimulation. It is also useful for high-throughput screening of potential CRHR2-targeting drugs.
A: Investigating CRHR2 in the Human CRHR2-tGFP Stable Cell Line-CHO can contribute to our understanding of its role in stress-related disorders and diseases like anxiety, depression, and post-traumatic stress disorder. This knowledge can lead to the development of targeted therapies or interventions.
A: The stability of tGFP expression in the Human CRHR2-tGFP Stable Cell Line-CHO can vary depending on various factors, including the passage number, culture conditions, and cell handling techniques. It is crucial to monitor and maintain the cells properly to ensure consistent tGFP expression.
A: Some limitations may include the potential for genetic drift in stable cell lines and the need for consistent maintenance. To mitigate these challenges, regular monitoring, cryopreservation of early passages, and stringent quality control are recommended.
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