Cat.No. : CSC-RG0009 Host Cell: HEK293
| Cat. No. | CSC-RG0009 |
| Background | Corticotropin Releasing Hormone Repceptor 2 is the gene that encodes a protein also known as CRHR-2. The Corticotropin Releasing Hormone Receptor family is a group of Gcoupled receptors that shows high affinity for Corticotropin Releasing Hormone (CRH). CRH is a polypeptide hormone and neurotransmitter synthesized in the hypothalamus that is the principal neuroregulator of the hypothalamic-pituitaryadrenocortical axis. CRH is involved in the stress response and the stimulation of the pituitary synthesis of ACTH (Adrenocorticotropic hormone). |
| Gene | CRHR2 |
| Gene Species | Homo sapiens (Human) |
| Alias | CRHR2, CRF-RB, CRF2, CRFR2, HM-CRF |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The HEK293 cell line, which has been stably transfected with the CRHR2 gene, forms the Human CRHR2 Stable Cell Line-HEK293. This cell line plays a crucial role in studying stress responses and related diseases such as depression, anxiety, and schizophrenia. CRHR2 is a key receptor in the brain that responds to stress hormones, cortisol. With this cell line, researchers can model and investigate the activity changes of CRHR2 under stressful conditions and how these changes affect cellular functions and signal transduction pathways.
A: Researchers can evaluate the affinity and effects of potential drugs on CRHR2 by conducting drug screening and testing on the Human CRHR2 Stable Cell Line-HEK293. This is significant for developing drugs targeting CRHR2, such as antidepressants. Additionally, this cell line can be used to study the regulatory effects of drugs on the CRHR2 signaling pathway, providing insights into drug side effects and tolerance.
A: The Human CRHR2 Stable Cell Line-HEK293 is crucial for understanding the role of CRHR2 in the mechanisms of neurodegenerative diseases. For example, CRHR2 may be involved in regulating neuronal survival and death in Alzheimer's and Parkinson's diseases. With this cell line, scientists can investigate the expression and function of CRHR2 in these disease models, as well as potential therapeutic interventions.
A: This cell line allows scientists to study the effects of CRHR2 gene mutations on cellular functions in a controlled environment. By comparing the expression and activity of wild-type and mutant CRHR2, researchers can better understand how these mutations affect CRHR2 signaling and cellular responses, which is significant for the molecular pathology of genetic mental illnesses.
A: The Human CRHR2 Stable Cell Line-HEK293 provides a powerful tool for studying the regulation of the CRHR2 signaling pathway. Researchers can explore the downstream effects of CRHR2 activation, including the activation of intracellular signaling molecules, changes in gene expression, and regulation of cellular behavior. This is crucial for revealing the mechanisms of CRHR2's role in physiological and pathological processes.
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