Cat.No. : CSC-RG0001 Host Cell: HEK293
| Cat. No. | CSC-RG0001 |
| Description | This cell line is engineered to overexpress human cannabinoid receptor 2 (CNR2). |
| Gene | CNR2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CNR2 Stable Cell Line - HEK293 offers several advantages for endocannabinoid research. Firstly, HEK293 cells are widely used due to their high transfection efficiency, which facilitates the introduction of the CNR2 gene, allowing for stable expression of the CB2 receptor. Secondly, these cells are easy to culture and maintain, providing a consistent and reproducible model for experiments. Thirdly, the use of HEK293 cells allows for a better control over experimental variables, as they are less prone to native variation in receptor expression compared to primary cells.
A: The Human CNR2 Stable Cell Line - HEK293 enables researchers to study the CB2 receptor's signaling mechanisms in a controlled environment. By stably expressing the CB2 receptor, researchers can investigate ligand binding, receptor activation, and downstream signaling events in a cell line that is otherwise devoid of this receptor. This provides a clear and direct way to study the CB2 receptor's function without interference from other receptors or cellular processes.
A: The Human CNR2 Stable Cell Line - HEK293 can be utilized in high-throughput screening assays to identify novel compounds that modulate CB2 receptor activity. This cell line can serve as a platform for testing the efficacy and safety of potential therapeutic agents targeting the CB2 receptor, which could be beneficial in the treatment of various conditions, including pain, inflammation, and neurological disorders.
A: The Human CNR2 Stable Cell Line - HEK293 provides a tool for studying the immunomodulatory effects of CB2 receptors. By stably expressing the CB2 receptor, researchers can investigate how it influences immune cell functions, such as cytokine production and immune cell migration, which are critical in understanding the role of the endocannabinoid system in the immune response.
A: The Human CNR2 Stable Cell Line - HEK293 allows for the examination of receptor desensitization and internalization processes by providing a controlled environment where these events can be monitored in response to ligand stimulation. This can help researchers understand the mechanisms of CB2 receptor regulation and how they may be affected by various factors, such as chronic ligand exposure.
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