Human CNR2 Stable Cell Line - HEK293

Name: Human CNR2 Stable Cell Line - HEK293
SKU: CSC-RG0001
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0001

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0001
Description	This cell line is engineered to overexpress human cannabinoid receptor 2 (CNR2).
Target Gene	CNR2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CNR2 cannabinoid receptor 2 (macrophage) [ Homo sapiens ]
Gene Symbol	CNR2
Synonyms	CNR2; cannabinoid receptor 2 (macrophage); cannabinoid receptor 2; CB2; testis-dominant CNR2 isoform CB2; CX5; CB-2;
GeneID	1269
Uni ProtID	P34972
mRNA Refseq	BC069722
Chromosome Location	1p
Function	cannabinoid receptor activity; receptor activity; signal transducer activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	605051

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Customer Q&As

What are the specific advantages of using the Human CNR2 Stable Cell Line - HEK293 for studying the endocannabinoid system?

A: The Human CNR2 Stable Cell Line - HEK293 offers several advantages for endocannabinoid research. Firstly, HEK293 cells are widely used due to their high transfection efficiency, which facilitates the introduction of the CNR2 gene, allowing for stable expression of the CB2 receptor. Secondly, these cells are easy to culture and maintain, providing a consistent and reproducible model for experiments. Thirdly, the use of HEK293 cells allows for a better control over experimental variables, as they are less prone to native variation in receptor expression compared to primary cells.

How does the Human CNR2 Stable Cell Line - HEK293 contribute to the understanding of CB2 receptor signaling pathways?

A: The Human CNR2 Stable Cell Line - HEK293 enables researchers to study the CB2 receptor's signaling mechanisms in a controlled environment. By stably expressing the CB2 receptor, researchers can investigate ligand binding, receptor activation, and downstream signaling events in a cell line that is otherwise devoid of this receptor. This provides a clear and direct way to study the CB2 receptor's function without interference from other receptors or cellular processes.

What are the potential applications of the Human CNR2 Stable Cell Line - HEK293 in drug discovery for the endocannabinoid system?

A: The Human CNR2 Stable Cell Line - HEK293 can be utilized in high-throughput screening assays to identify novel compounds that modulate CB2 receptor activity. This cell line can serve as a platform for testing the efficacy and safety of potential therapeutic agents targeting the CB2 receptor, which could be beneficial in the treatment of various conditions, including pain, inflammation, and neurological disorders.

How does the Human CNR2 Stable Cell Line - HEK293 help in elucidating the role of CB2 receptors in immune regulation?

A: The Human CNR2 Stable Cell Line - HEK293 provides a tool for studying the immunomodulatory effects of CB2 receptors. By stably expressing the CB2 receptor, researchers can investigate how it influences immune cell functions, such as cytokine production and immune cell migration, which are critical in understanding the role of the endocannabinoid system in the immune response.

How does the Human CNR2 Stable Cell Line - HEK293 support the investigation of CB2 receptor desensitization and internalization?

A: The Human CNR2 Stable Cell Line - HEK293 allows for the examination of receptor desensitization and internalization processes by providing a controlled environment where these events can be monitored in response to ligand stimulation. This can help researchers understand the mechanisms of CB2 receptor regulation and how they may be affected by various factors, such as chronic ligand exposure.

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