Cat.No. : CSC-RG0023 Host Cell: CHO-K1
| Cat. No. | CSC-RG0023 |
| Background | The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dosedependent, stereoselective, and pertussis toxinsensitive manner. These proteins have been found to be involved in the cannabinoidinduced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. |
| Gene | CNR2 |
| Gene Species | Homo sapiens (Human) |
| Alias | CNR2, CB2, CX5, CB-2 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human CNR2-tGFP Stable Cell Line-CHO is a cell line that stably expresses the human CNR2 gene encoding Cannabinoid Receptor 2 (CNR2) along with green fluorescent protein (tGFP). CHO stands for Chinese Hamster Ovary, which is a commonly used mammalian cell line. The primary feature of this cell line is its stable expression of CNR2 and tGFP, allowing researchers to track the expression and localization of CNR2.
A: The CNR2 gene encodes Cannabinoid Receptor 2 (CNR2), also known as CB2 receptor, which is a G-protein-coupled receptor and part of the cannabinoid receptor family. CB2 receptors are primarily found in immune cells, peripheral tissues, and microglial cells in the central nervous system. Its main functions involve immune modulation, inflammation regulation, and interaction with cannabinoid-like compounds. CB2 receptors play a crucial role in immune and inflammatory responses, making Human CNR2-tGFP Stable Cell Line-CHO valuable for studying the function and regulatory mechanisms of CB2 receptors.
A: CHO cells are a commonly used mammalian host cell line with several advantages that make them an ideal choice for expressing foreign genes. CHO cells are easy to culture and maintain, and there is a wealth of experience in engineering them. Additionally, CHO cells are highly efficient and stable in producing recombinant proteins, which is crucial for stably expressing CNR2 and tGFP. CHO cells also have less background interference, allowing for more accurate research into the function and dynamics of CNR2 and tGFP in cells.
A: To maintain the stability of the Human CNR2-tGFP Stable Cell Line-CHO, the following measures are typically taken:
Regular Culturing: The cells need to be regularly cultured and passaged to keep them in a healthy and growing state.
Antibiotic Selection: Continued use of a culture medium containing antibiotics to ensure only cells with the CNR2 and tGFP genes remain viable.
Backup Freezing: Periodically freezing backup stocks of cells to prevent the loss or contamination of the cell line.
Regular Testing: Regularly assessing the expression levels of CNR2 and tGFP to ensure their stability.
A: The Human CNR2-tGFP Stable Cell Line-CHO holds potential applications in inflammation research. Due to the role of CNR2 receptors in immune regulation and inflammatory responses, this cell line can be used for research in the following areas:
Investigating the mechanisms by which CNR2 receptors regulate inflammatory responses.
Assessing the impact of cannabinoid-like compounds on inflammation and immune cells.
Drug screening and evaluation for anti-inflammatory therapies targeting CNR2 receptors.
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