Cat.No. : CSC-RG0008 Host Cell: HEK293
| Cat. No. | CSC-RG0008 |
| Background | This gene encodes a high affinity receptor for the peptide hormone Calcitonin and belongs to a subfamily of seven transmembrane spanning G protein coupled receptors. The protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis. Alternate splicing results in multiple transcript variants. |
| Gene | CALCR |
| Gene Species | Homo sapiens (Human) |
| Alias | CALCR, CRT, CT-R, CTR, CTR1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CALCR Stable Cell Line-HEK293 serves as a model system for studying signal transduction mediated by the Calcitonin receptor (CALCR). By expressing CALCR in these cells, scientists can investigate how changes in downstream signaling pathways occur upon receptor activation and how these changes influence cellular behaviors.
A: The Human CALCR Stable Cell Line-HEK293 can assist researchers in developing new drugs targeting CALCR through drug screening experiments. These cells can be used in high-throughput screening platforms to evaluate the activity of compounds on CALCR, thereby revealing potential drug candidates.
A: The Human CALCR Stable Cell Line-HEK293 can be used to study the endogenous regulation mechanisms of CALCR within the cell, including its expression levels, subcellular localization, and interactions with other molecules.
A: The Human CALCR Stable Cell Line-HEK293 is crucial for studying the role of CALCR in diseases. By simulating disease states in these cells, scientists can explore the role of CALCR in disease progression and potential therapeutic targets.
A: The Human CALCR Stable Cell Line-HEK293 assists in optimizing drug design by providing direct evidence of drug action. By analyzing how drugs affect the function of CALCR, researchers can adjust the chemical structure of drugs to improve their potency and selectivity.
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