Human CALCR-tGFP Stable Cell Line-CHO-K1

Name: Human CALCR-tGFP Stable Cell Line-CHO-K1
SKU: CSC-RG0031
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0031

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0031
Background	This gene encodes a high affinity receptor for the peptide hormone Calcitonin and belongs to a subfamily of seven transmembrane- spanning G protein coupled receptors. The protein is involved in maintaining calcium homeostasis and in regulating osteoclast- mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis. Alternate splicing results in multiple transcript variants.
Target Gene	CALCR
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCALCR-tGFP
Alias	CALCR, CTR, CRT, CT-R, CTR1
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CALCR calcitonin receptor [ Homo sapiens ]
Gene Symbol	CALCR
Synonyms	CALCR; calcitonin receptor; CTR; CRT; CT-R; CTR1;
GeneID	799
Uni ProtID	P30988
mRNA Refseq	BC069611
Chromosome Location	7q21.3
Function	calcitonin binding; NOT calcitonin binding; calcitonin binding; calcitonin receptor activity; calcitonin receptor activity; protein binding; protein transporter activity; receptor activity; receptor activity; signal transducer activity;
Pathway	Calcitonin-like ligand receptors, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class B Secretin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
MIM	114131

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Customer Q&As

What role does the GFP tag play in the Human CALCR-tGFP Stable Cell Line-CHO for tracking receptor dynamics?

A: The green fluorescent protein (GFP) tag attached to the calcitonin receptor (CALCR) in this cell line serves as a fluorescent marker. This enables researchers to track the movement and localization of the receptor within the cell under a fluorescence microscope. The GFP tag does not interfere with the receptor's function, allowing for the study of receptor trafficking, activation, and deactivation processes in response to ligand binding.

How does the Human CALCR-tGFP Stable Cell Line-CHO facilitate the study of calcitonin receptor signaling pathways?

A: The Human CALCR-tGFP Stable Cell Line-CHO (Chinese Hamster Ovary) is engineered to express the calcitonin receptor (CALCR) tagged with the green fluorescent protein (tGFP). This allows researchers to visualize and monitor the activation of the receptor in real-time, providing a powerful tool for studying signaling pathways. By activating the receptor with its ligand, calcitonin, researchers can observe the intracellular distribution of the receptor and its associated tGFP fluorescence, which can indicate receptor activation, internalization, and downstream signaling events.

How can the Human CALCR-tGFP Stable Cell Line-CHO be used to investigate the pharmacology of calcitonin analogs?

A: By exposing the Human CALCR-tGFP Stable Cell Line-CHO to various calcitonin analogs, researchers can assess their efficacy and selectivity. The fluorescence changes associated with receptor activation can be quantified, providing a readout for the potency and specificity of different analogs. This can help in the development of new therapeutic agents targeting the calcitonin receptor.

In what applications can the Human CALCR-tGFP Stable Cell Line-CHO be utilized for drug screening?

A: The cell line can be used in high-throughput screening (HTS) assays to identify compounds that modulate the activity of the calcitonin receptor. By monitoring changes in tGFP fluorescence, researchers can screen large libraries of compounds for their ability to activate or inhibit the receptor, which can lead to the discovery of novel therapeutics.

How does the Human CALCR-tGFP Stable Cell Line-CHO contribute to the understanding of receptor desensitization?

A: By continuously stimulating the cells with calcitonin, researchers can observe the receptor's response over time. The decrease in tGFP fluorescence can indicate receptor desensitization, a process where the receptor becomes less responsive to its ligand due to prolonged stimulation. This can provide insights into the mechanisms of receptor desensitization and resensitization.

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