Cat.No. : CSC-RG0031 Host Cell: CHO-K1
| Cat. No. | CSC-RG0031 |
| Background | This gene encodes a high affinity receptor for the peptide hormone Calcitonin and belongs to a subfamily of seven transmembrane- spanning G protein coupled receptors. The protein is involved in maintaining calcium homeostasis and in regulating osteoclast- mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis. Alternate splicing results in multiple transcript variants. |
| Gene | CALCR |
| Gene Species | Homo sapiens (Human) |
| Alias | CALCR, CTR, CRT, CT-R, CTR1 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The green fluorescent protein (GFP) tag attached to the calcitonin receptor (CALCR) in this cell line serves as a fluorescent marker. This enables researchers to track the movement and localization of the receptor within the cell under a fluorescence microscope. The GFP tag does not interfere with the receptor's function, allowing for the study of receptor trafficking, activation, and deactivation processes in response to ligand binding.
A: The Human CALCR-tGFP Stable Cell Line-CHO (Chinese Hamster Ovary) is engineered to express the calcitonin receptor (CALCR) tagged with the green fluorescent protein (tGFP). This allows researchers to visualize and monitor the activation of the receptor in real-time, providing a powerful tool for studying signaling pathways. By activating the receptor with its ligand, calcitonin, researchers can observe the intracellular distribution of the receptor and its associated tGFP fluorescence, which can indicate receptor activation, internalization, and downstream signaling events.
A: By exposing the Human CALCR-tGFP Stable Cell Line-CHO to various calcitonin analogs, researchers can assess their efficacy and selectivity. The fluorescence changes associated with receptor activation can be quantified, providing a readout for the potency and specificity of different analogs. This can help in the development of new therapeutic agents targeting the calcitonin receptor.
A: The cell line can be used in high-throughput screening (HTS) assays to identify compounds that modulate the activity of the calcitonin receptor. By monitoring changes in tGFP fluorescence, researchers can screen large libraries of compounds for their ability to activate or inhibit the receptor, which can lead to the discovery of novel therapeutics.
A: By continuously stimulating the cells with calcitonin, researchers can observe the receptor's response over time. The decrease in tGFP fluorescence can indicate receptor desensitization, a process where the receptor becomes less responsive to its ligand due to prolonged stimulation. This can provide insights into the mechanisms of receptor desensitization and resensitization.
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