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Human AVPR1B Stable Cell Line-HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RG1227

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RG1227
Description This cell line is engineered to stably overexpress Human AVPR1B in HEK293 cells.
Target Gene AVPR1B
Gene Species Homo sapiens (Human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Research on the mechanisms of GPCR-related diseases

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name AVPR1B arginine vasopressin receptor 1B [ Homo sapiens ]
Gene Symbol AVPR1B
Synonyms AVPR3
Gene Description arginine vasopressin receptor 1B
Gene ID 553
Uni Prot ID P47901
m RNA Refseq NM_000707.3
Protein Refseq NP_000698.1
Chromosome Location 1q32
Function peptide hormone binding; protein homodimerization activity; protein kinase C binding; vasopressin receptor activity;
Pathway Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem;
MIM 600264
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