Human AVPR1A-SNAP Stable Cell Line-HEK293

Name: Human AVPR1A-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0056
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0056

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0056
Background	The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis.
Target Gene	AVPR1A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuAVPR1A-SNAP
Alias	AVPR1A, AVPR1, V1aR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	AVPR1A arginine vasopressin receptor 1A [ Homo sapiens ]
Gene Symbol	AVPR1A
Synonyms	V1aR; AVPR1; AVPR V1a
GeneID	552
Uni ProtID	P37288
mRNA Refseq	NM_000706.4
Protein Refseq	NP_000697.1
Chromosome Location	12q14-q15
Function	V1A vasopressin receptor binding; peptide hormone binding; protein binding; protein kinase C binding; vasopressin receptor activity;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem;
MIM	600821

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Customer Q&As

What is the significance of the Human AVPR1A-SNAP Stable Cell Line-HEK293 in the field of neuroscience research?

A: The Human AVPR1A-SNAP Stable Cell Line-HEK293 is significant for neuroscience research as it allows for the study of the Arginine Vasopressin 1A Receptor (AVPR1A), which is involved in various physiological processes such as water balance and social behavior. The SNAP tag enables the visualization and tracking of AVPR1A within the cell, providing insights into its subcellular localization and trafficking.

How does the Human AVPR1A-SNAP Stable Cell Line-HEK293 contribute to the understanding of water homeostasis?

A: This cell line is instrumental in studying the role of AVPR1A in water homeostasis. AVPR1A is a receptor for vasopressin, which regulates water reabsorption in the kidneys. By observing the function of AVPR1A in this cell line, researchers can gain insights into how dysregulation of this receptor might contribute to conditions like diabetes insipidus.

What are the potential applications of the Human AVPR1A-SNAP Stable Cell Line-HEK293 in drug discovery?

A: This cell line can be used for high-throughput screening of compounds that interact with AVPR1A. By monitoring changes in receptor activity, researchers can identify potential drug candidates for conditions related to AVPR1A dysfunction, such as urinary disorders or neurological diseases.

How does the Human AVPR1A-SNAP Stable Cell Line-HEK293 aid in the study of social behavior and its neurological basis?

A: AVPR1A has been implicated in social bonding and behavior. The stable cell line provides a platform to study the molecular mechanisms underlying these behaviors, which could lead to a better understanding of social disorders and the development of therapeutic strategies.

How does the Human AVPR1A-SNAP Stable Cell Line-HEK293 facilitate the study of AVPR1A signaling pathways?

A: By expressing AVPR1A with a SNAP tag, researchers can use various biochemical and molecular techniques to probe the receptor's signaling cascades. This includes assessing the activation of downstream effectors and the involvement of intracellular signaling molecules.

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