Cat.No. : CSC-RG0056 Host Cell: HEK293
| Cat. No. | CSC-RG0056 |
| Background | The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. |
| Gene | AVPR1A |
| Gene Species | Homo sapiens (Human) |
| Alias | AVPR1A, AVPR1, V1aR |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human AVPR1A-SNAP Stable Cell Line-HEK293 is significant for neuroscience research as it allows for the study of the Arginine Vasopressin 1A Receptor (AVPR1A), which is involved in various physiological processes such as water balance and social behavior. The SNAP tag enables the visualization and tracking of AVPR1A within the cell, providing insights into its subcellular localization and trafficking.
A: This cell line is instrumental in studying the role of AVPR1A in water homeostasis. AVPR1A is a receptor for vasopressin, which regulates water reabsorption in the kidneys. By observing the function of AVPR1A in this cell line, researchers can gain insights into how dysregulation of this receptor might contribute to conditions like diabetes insipidus.
A: This cell line can be used for high-throughput screening of compounds that interact with AVPR1A. By monitoring changes in receptor activity, researchers can identify potential drug candidates for conditions related to AVPR1A dysfunction, such as urinary disorders or neurological diseases.
A: AVPR1A has been implicated in social bonding and behavior. The stable cell line provides a platform to study the molecular mechanisms underlying these behaviors, which could lead to a better understanding of social disorders and the development of therapeutic strategies.
A: By expressing AVPR1A with a SNAP tag, researchers can use various biochemical and molecular techniques to probe the receptor's signaling cascades. This includes assessing the activation of downstream effectors and the involvement of intracellular signaling molecules.
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