Human AVPR1A Stable Cell Line-HEK293

Name: Human AVPR1A Stable Cell Line-HEK293
SKU: CSC-RG0018

Cat.No. : CSC-RG0018 Host Cell: HEK293

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RG0018
Gene	AVPR1A
Gene Species	Homo sapiens (Human)
Alias	AVPR1A, AVPR V1a, AVPR1, V1aR
Host Cell	HEK293
Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

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Customer Reviews

The activity of Arginine Vasopressin receptor 1A (AVPR1A) is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. Arginine Vasopressin 1A receptor belongs to the same subfamily of G-protein coupled receptors of AVPR1B, V2R and Oxytocin receptors. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. V1A is coupled to Gq/11 proteins and is found at high density on smooth muscle cells. It is involved in regulation of blood pressure through arterial vasoconstriction and has been found to stimulate VEGF secretion. AVPR1A is widely expressed in the brain and it has been implicated in social behaviours, including affiliation and attachment and related to diseases as autism.

Customer Q&As

What is the role of the Human AVPR1A Stable Cell Line-HEK293 in studying the function of the vasopressin receptor?

A: The Human AVPR1A Stable Cell Line-HEK293, established by stably expressing the human vasopressin receptor A (AVPR1A) in HEK293 cells, plays a crucial role in studying the function of AVPR1A in water balance regulation, kidney function, and related diseases such as diabetes insipidus. Scientists can simulate and investigate the activity changes of AVPR1A receptors and how these changes affect cellular functions and signal transduction pathways.

How does the Human AVPR1A Stable Cell Line-HEK293 assist in drug screening to discover new vasopressin receptor modulators?

A: By conducting drug screening on the Human AVPR1A Stable Cell Line-HEK293, researchers can evaluate the affinity and effects of potential drugs on AVPR1A receptors. This cell line provides a platform for identifying and optimizing compounds that can affect AVPR1A activity, which is significant for developing new drugs to treat diabetes insipidus and other related conditions.

How is the Human AVPR1A Stable Cell Line-HEK293 applied in studying the role of AVPR1A receptors in kidney diseases?

A: The Human AVPR1A Stable Cell Line-HEK293 can be used to study the role of AVPR1A receptors in kidney diseases, particularly in regulating urine concentration and water reabsorption. This cell line helps scientists understand the molecular mechanisms of AVPR1A receptors in kidney dysfunction, which has potential value for developing new strategies to treat kidney diseases.

How is the Human AVPR1A Stable Cell Line-HEK293 applied in studying the role of AVPR1A receptors in water balance regulation?

A: The Human AVPR1A Stable Cell Line-HEK293 is crucial for studying the role of AVPR1A receptors in water balance regulation. This cell line allows scientists to simulate and observe the activity of AVPR1A receptors in regulating the body's water balance, which is significant for understanding the pathophysiology of water balance disorders.

How is the Human AVPR1A Stable Cell Line-HEK293 applied in studying the role of AVPR1A receptors in cardiovascular diseases?

A: The Human AVPR1A Stable Cell Line-HEK293 can be used to study the role of AVPR1A receptors in cardiovascular diseases, particularly in regulating vascular constriction and blood pressure. This cell line helps scientists understand the molecular mechanisms of AVPR1A receptors in cardiovascular diseases, which has potential value for developing new treatments for cardiovascular conditions.

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