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rAAV-EF1a-DIO-EGFP-F2A-TVA-T2A-RVG

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-56

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Virus Particles Information

Cat. No. RABVH-56
Description Cre-dependent all-in-one AAV helper co-expressing EGFP, TVA and RVG via F2A/T2A under EF1a. TVA makes Cre-positive neurons permissive to EnvA-pseudotyped G-deleted rabies virus, while the co-expressed RVG complements its G deletion to permit a single retrograde transsynaptic step. EGFP marks the starter population in a single-injection, Cre-specific monosynaptic tracing strategy.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing EGFP, TVA, and RVG via F2A/T2A under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing
  2. F2A and T2A for tricistronic expression
  3. EGFP for green starter visualization
  4. DIO restricts all components to Cre+ populations
  5. Mapping direct presynaptic inputs with simplified single-injection helper
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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