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rAAV-hSyn-H2B-EGFP-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-27

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Virus Particles Information

Cat. No. RABVH-27
Description Constitutive neuronal AAV helper co-expressing nuclear H2B-EGFP and TVA via T2A under the neuron-specific hSyn promoter. TVA makes transduced neurons permissive to EnvA-pseudotyped G-deleted rabies virus, and nuclear EGFP marks them with high contrast. Paired with an RVG helper, it enables retrograde monosynaptic tracing without any recombinase requirement.
Product Type Adeno-associated virus
Reporter EGFP
Helper Function AAV helper expressing nuclear-localized EGFP and TVA under neuronal hSyn promoter
Applications
  1. Co-injection with RV-EnvA-ΔG for constitutive monosynaptic retrograde tracing in neurons
  2. hSyn promoter restricts expression to neurons
  3. H2B fusion confines EGFP to nucleus for unambiguous starter cell identification
  4. T2A enables bicistronic expression
  5. Mapping direct presynaptic inputs to neurons in a defined brain region
  6. No Cre/Flp requirement — uses hSyn for neuronal specificity
Promoter Neuron-specific Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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