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rAAV-EF1a-DIO-EGFP-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-22

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Virus Particles Information

Cat. No. RABVH-22
Description Cre-dependent AAV helper co-expressing EGFP and TVA from a single T2A-linked transcript under EF1a. EGFP labels Cre-positive starter neurons in green, and TVA makes them permissive to EnvA-pseudotyped G-deleted rabies virus. With RVG complementation, the virus crosses one synapse retrogradely to reveal the direct presynaptic inputs of the targeted population.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing EGFP and TVA under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with green starter labeling
  2. EGFP and TVA co-expressed via T2A
  3. DIO restricts expression to Cre+ populations
  4. Mapping direct presynaptic inputs to Cre-defined populations
  5. Standard monosynaptic retrograde tracing protocol
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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