rAAV-EF1a-fDIO-TVA(E66T)-mCherry

Name: rAAV-EF1a-fDIO-TVA(E66T)-mCherry
SKU: RABVH-4
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RABVH-4

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Virus Particles Information

Cat. No.	RABVH-4
Description	Flp-dependent AAV helper expressing a low-affinity TVA(E66T) mutant fused to mCherry under EF1a. The weakened receptor reduces background infection of non-starter cells while retaining efficient entry of EnvA-pseudotyped G-deleted rabies virus in Flp-expressing neurons; mCherry marks these starter cells for one-step retrograde monosynaptic input mapping.
Product Type	Adeno-associated virus
Reporter	mCherry
Expression Mechanism	Flp-dependent
Helper Function	AAV helper expressing low-affinity mutant TVA(E66T) fused with mCherry under Flp-dependent control
Applications	Co-injection with RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing with low-affinity TVA TVA(E66T) has lower affinity for EnvA, reducing background/pseudo-negative starter cell results mCherry fused to TVA(E66T) for visualization fDIO restricts expression to Flp+ populations Improved starter cell identification in monosynaptic retrograde tracing (Miyamichi et al. 2013) Reduces pseudo-negative starter cells caused by excessive TVA affinity
Promoter	Broad-spectrum Promoter
Size	Custom
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Dry ice

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