rAAV-EF1a-fDIO-TVA-P2A-NLS-dTomato

Name: rAAV-EF1a-fDIO-TVA-P2A-NLS-dTomato
SKU: RABVH-2
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RABVH-2

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Virus Particles Information

Cat. No.	RABVH-2
Description	Flp-dependent AAV helper co-expressing TVA and nuclear-localized dTomato (via P2A) under EF1a. TVA renders Flp-expressing neurons permissive to EnvA-pseudotyped G-deleted rabies virus, and the nuclear dTomato signal gives crisp, single-cell resolution of starter cells. Used with an RVG helper, it supports Flp-based retrograde monosynaptic input mapping.
Product Type	Adeno-associated virus
Reporter	Tomato
Expression Mechanism	Flp-dependent
Helper Function	AAV helper expressing TVA and nuclear-localized dTomato under Flp-dependent control
Applications	Co-injection with RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing with nuclear starter labeling NLS (nuclear localization signal) confines dTomato to nucleus for unambiguous starter cell identification P2A self-cleaving peptide enables bicistronic expression Nuclear-localized reporter overcomes dense cytoplasmic labeling in starter populations Mapping direct presynaptic inputs to Flp-defined populations
Promoter	Broad-spectrum Promoter
Size	Custom
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Dry ice

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