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rAAV-CaMKIIa-H2B-mCherry-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-32

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Virus Particles Information

Cat. No. RABVH-32
Description Constitutive AAV helper co-expressing nuclear H2B-mCherry and TVA via T2A under the CaMKIIa promoter, limiting expression to excitatory neurons. TVA renders these cells permissive to EnvA-pseudotyped G-deleted rabies virus, and nuclear mCherry marks them in red. With an RVG helper, the direct presynaptic inputs of excitatory neurons can be mapped retrogradely.
Product Type Adeno-associated virus
Reporter mCherry
Helper Function AAV helper expressing nuclear mCherry and TVA in excitatory neurons
Applications
  1. Co-injection with RV-EnvA-ΔG for constitutive monosynaptic retrograde tracing in excitatory neurons
  2. CaMKIIα promoter restricts to excitatory pyramidal neurons
  3. H2B-mCherry for unambiguous starter cell identification
  4. T2A enables bicistronic expression
  5. Mapping direct presynaptic inputs to excitatory projection neurons
Promoter Neuron-specific Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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