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rAAV-EF1a-DIO-mCherry-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-17

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Virus Particles Information

Cat. No. RABVH-17
Description Cre-dependent AAV helper co-expressing mCherry and TVA through a T2A self-cleaving peptide under EF1a. mCherry identifies Cre-expressing starter neurons in red, while TVA makes them selectively permissive to EnvA-pseudotyped G-deleted rabies virus. Combined with an RVG helper, single-synapse retrograde spread reveals the direct presynaptic inputs of the Cre-defined population.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing mCherry and TVA under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with red starter labeling
  2. mCherry and TVA co-expressed via T2A
  3. DIO restricts expression to Cre+ populations
  4. Dual-color experiments with EGFP-labeled rabies virus
  5. Mapping direct presynaptic inputs to Cre-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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