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rAAV-EF1a-fDIO(W)-RVG

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-40

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Virus Particles Information

Cat. No. RABVH-40
Description Flp-dependent AAV helper expressing rabies virus glycoprotein (RVG) from a WPRE-enhanced cassette under EF1a. The WPRE increases RVG transcript stability and expression in Flp-positive neurons, improving complementation of G-deleted rabies virus and the efficiency of a single retrograde transsynaptic step. Used with a TVA helper for Flp-specific monosynaptic tracing.
Product Type Adeno-associated virus
Expression Mechanism Flp-dependent
Helper Function AAV helper expressing RVG with WPRE enhancer under Flp-dependent control
Applications
  1. Co-injection with TVA helper AAV and RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing
  2. WPRE enhances RVG expression for stronger trans-complementation
  3. fDIO(W) restricts to Flp+ populations with enhanced levels
  4. Mapping direct presynaptic inputs with higher efficiency
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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