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rAAV-EF1a-DIO-H2B-BFP-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-21

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Virus Particles Information

Cat. No. RABVH-21
Description Cre-dependent AAV helper co-expressing nuclear H2B-BFP and TVA via T2A under EF1a. The nuclear blue reporter marks Cre-expressing starter neurons without occupying the green or red channels, while TVA allows selective infection by EnvA-pseudotyped G-deleted rabies virus. This three-colour-compatible design supports Cre-defined monosynaptic retrograde tracing alongside other circuit labels.
Product Type Adeno-associated virus
Reporter BFP
Expression Mechanism Cre-dependent
Helper Function AAV helper expressing nuclear-localized BFP and TVA under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with nuclear blue starter labeling
  2. H2B fusion confines BFP to nucleus
  3. T2A enables bicistronic expression
  4. DIO restricts expression to Cre+ populations
  5. Triple-color experiments with EGFP and mCherry rabies tracers
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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