Human PTH2R Stable Cell Line - HEK293

The parathyroid hormone 2 receptor (PTH2R) is a G protein-coupled receptor (GPCR) encoded by the human PTH2R gene. This receptor plays a crucial role in calcium homeostasis and bone metabolism by binding to parathyroid hormone (PTH) and its endogenous ligand, tuberous infundiposide (TIP39). PTH2R is primarily distributed in the brain, pancreas, and testes, participating in neuroendocrine regulation, insulin secretion, and reproductive function. Activation of PTH2R triggers intracellular signaling pathways, including cAMP production, thereby regulating various physiological processes such as neuronal excitability, hormone release, and bone remodeling. Mutations or dysfunction of PTH2R are associated with diseases such as osteoporosis and neuropsychiatric disorders, making it an important target for therapeutic research.

The human PTH2R stable cell line HEK293 is a genetically engineered cell model derived from human embryonic kidney 293 (HEK293) cells, designed to stably express the human PTH2R gene. This cell line is a powerful tool for studying receptor biology, ligand-receptor interactions, and signal transduction mechanisms, and can be performed under controlled in vitro conditions. HEK293 cells are favored for their high transfection efficiency, vigorous growth, and compatibility with biochemical analyses such as calcium ion flux assays, cAMP accumulation, and GPCR internalization studies. Stable integration of PTH2R ensures consistent receptor expression during passage, thus eliminating the variability introduced by transient transfection. Researchers utilize this cell line for high-throughput screening of PTH2R agonists and antagonists, drug development for bone diseases, and mechanistic studies of PTH2R-related pathologies. Furthermore, this cell line can be used for the functional characterization of receptor mutants and the validation of pharmacological compounds, providing a reliable platform for advancing therapeutic strategies.