Human HTR4 Stable Cell Line - HEK293

Name: Human HTR4 Stable Cell Line - HEK293
SKU: CSC-RG01857
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG01857

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG01857
Description	This cell line is engineered to stably overexpress human HTR4 in HEK293 cells.
Product Type	Stable cell line constitutively expressing human kinase gene(s)
Target Gene	HTR4
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	HEK293 stable cell lines expressing GPCR genes have a wide range of uses in basic research and drug discovery. 1) High - throughput Screening: These cell lines are widely used in high - throughput screening assays to identify new ligands for GPCRs. By exposing the cells to large libraries of small molecules or other compounds and monitoring the resulting cellular responses, potential drug candidates can be rapidly identified. 2) Drug Target Validation: They provide a valuable tool for validating GPCRs as drug targets. Researchers can study the effects of specific drugs or genetic manipulations on the GPCR - expressing cells to assess the functional significance of the GPCR in a particular biological process. This helps to determine whether targeting a specific GPCR is likely to have therapeutic benefits. 3) Pharmacological Characterization: HEK293 stable cell lines allow for the detailed pharmacological characterization of GPCR ligands. Parameters such as binding affinity, efficacy, and potency can be determined by measuring the ligand - induced responses in these cells. This information is crucial for optimizing the design of new drugs and understanding their mechanism of action.
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) Analysis of GPCR function by cAMP assay 3) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

HTR4

Background

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]

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