Human CD276 Stable Cell Line - MC38

Name: Human CD276 Stable Cell Line - MC38
SKU: CSC-RO0917
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0917

Host Cell : MC38 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0917
Description	This cell line is engineered to stably overexpress human CD276 (also named as B7H3).
Target Gene	CD276
Gene Species	Homo sapiens (Human)
Host Cell	MC38
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD276 CD276 molecule [ Homo sapiens ]
Gene Symbol	CD276
Synonyms	B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Gene Description	CD276 molecule
GeneID	80381
Uni ProtID	Q5ZPR3
mRNA Refseq	NM_025240.2
Protein Refseq	NP_079516.1
Chromosome Location	15q23-q24
Function	protein binding; receptor binding;
Pathway	Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem;
MIM	605715

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