Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0969 Host Cell : HEK293
| Cat. No. | CLOE-0969 |
| Introduction | This cell lysate over-expresses extracellular domain of human CD276 (Q5ZPR3-1) (Met 1-Thr 461) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified |
| Target Gene | CD276 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CD276 CD276 molecule [ Homo sapiens ] |
| Gene Symbol | CD276 |
| Synonyms | B7H3; B7-H3; B7RP-2; 4Ig-B7-H3 |
| Gene Description | CD276 molecule |
| GeneID | 80381 |
| Uni ProtID | Q5ZPR3 |
| mRNA Refseq | NM_025240.2 |
| Protein Refseq | NP_079516.1 |
| Chromosome Location | 15q23-q24 |
| Function | protein binding; receptor binding; |
| Pathway | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; |
| MIM | 605715 |
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