Cat.No. : CSC-RO0505 Host Cell: CHO-K1
| Cat. No. | CSC-RO0505 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Human CD24. |
| Gene | CD24 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: To generate the Human CD24 Stable Cell Line - CHO-K1, the process typically involves transfecting CHO-K1 cells with a plasmid containing the human CD24 gene along with a selectable marker gene, such as antibiotic resistance. Following transfection, cells are subjected to selective pressure using the appropriate antibiotic to allow only those cells containing the desired gene to survive. These surviving cells are then expanded and characterized to establish a stable cell line expressing human CD24.
A: During the establishment of the Human CD24 Stable Cell Line - CHO-K1, critical quality control measures are implemented at various stages. These measures may include verifying the expression of the CD24 gene through molecular techniques like PCR or Western blotting, confirming stable integration of the gene into the cell genome, assessing cell viability and growth characteristics, checking for genetic stability over passages, and ensuring consistent expression levels of CD24 over time.
A: CHO-K1 cells offer several advantages as hosts for stable cell line development. They have a well-characterized genome and are easily transfected, allowing for efficient gene delivery and stable integration. Additionally, CHO cells are widely used in biopharmaceutical production due to their capacity for high-density growth in suspension culture, robust protein expression machinery, and post-translational modification capabilities, which are crucial for producing biotherapeutic proteins like CD24.
A: The stability of CD24 expression in the Human CD24 Stable Cell Line - CHO-K1 is maintained through stringent culture conditions and regular monitoring of cell characteristics. Cells are cultured under consistent conditions with proper nutrient supply and controlled environment to minimize variations. Additionally, regular subculturing and monitoring for any changes in growth rate, morphology, or expression levels are conducted. Any deviations from the expected behavior are thoroughly investigated, and corrective actions, such as readjustment of culture conditions or gene editing if necessary, are implemented to ensure stable CD24 expression over passages.
A: The promoter region used to drive CD24 expression in the Human CD24 Stable Cell Line - CHO-K1 depends on the specific design of the expression vector. Commonly used promoters in CHO cell expression systems include the CMV (cytomegalovirus) promoter, SV40 (simian virus 40) promoter, and hybrid promoters such as the EF1α (elongation factor 1 alpha) promoter. These promoters drive robust and constitutive expression of the CD24 gene in CHO-K1 cells, ensuring sustained protein production.
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The Human CD24 Stable Cell Line - CHO-K1, through stable transfection specific to the human CD24 gene, ensures high-level and specific expression of the CD24 protein, providing an important tool for studying the role of CD24 in cell signaling and immune regulation.
Since the CD24 expressed by the Human CD24 Stable Cell Line - CHO-K1 is a key factor in the progression of various cancers and immune escape, this cell line can be used for high-throughput drug screening, accelerating the discovery of anticancer drugs.
The Human CD24 Stable Cell Line - CHO-K1 provides a stable platform for studying the expression changes of CD24 as a biomarker in different disease states, supporting early diagnosis and treatment monitoring.
Because the Human CD24 Stable Cell Line - CHO-K1 provides consistent CD24 expression levels across multiple experimental batches, it enhances the reproducibility and reliability of experiments, ensuring the accuracy of data.
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