Human CD24 Stable Cell Line - CHO-K1

Name: Human CD24 Stable Cell Line - CHO-K1
SKU: CSC-RO0505
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0505

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0505
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Human CD24.
Target Gene	CD24
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD24 CD24 molecule [ Homo sapiens ]
Gene Symbol	CD24
Synonyms	CD24A
Gene Description	CD24 molecule
Gene ID	100133941
Uni Prot ID	B6EC88
m RNA Refseq	NM_013230.2
Protein Refseq	NP_037362.1
Chromosome Location	6q21
Function	protein binding; protein kinase binding; protein tyrosine kinase activator activity; signal transducer activity;
Pathway	Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem;
MIM	600074

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The Human CD24 gene encodes a cell surface glycoprotein that is involved in cell-cell interactions and signal transduction. CD24 is expressed on various cell types, including B cells, neurons, and some cancer cells, and has been implicated in processes such as cell adhesion, migration, and immune response.

The CHO-K1 cell line, derived from Chinese hamster ovary cells, has been engineered to stably express the Human CD24 gene. This stable cell line serves as a model for studying the function of CD24 in cell biology and its role in disease. Researchers can use this cell line to investigate the effects of CD24 on cell behavior, its interaction with other cell surface molecules, and its potential as a biomarker or therapeutic target in cancer and other diseases.

Human CD24 Stable Cell Line - CHO-K1 is a cell line genetically engineered to stably express the CD24 gene, which encodes a sialoglycoprotein expressed on the surface of cells, involved in a wide range of cellular processes including proliferation, differentiation, and adhesion. The CD24 protein has been implicated in various diseases, including cancer, making this cell line a valuable tool for research in oncology, immunology, and cell biology. Below are three distinct applications for this cell line:

(1) Cancer Research and Drug Discovery:

The expression of CD24 has been correlated with the progression and prognosis of various types of cancer, such as breast, ovarian, and pancreatic cancers. The Human CD24 Stable Cell Line - CHO-K1 provides an ideal model for studying the role of CD24 in cancer biology. Researchers can use this cell line to investigate how CD24 contributes to tumor growth, metastasis, and resistance to chemotherapy. By screening compounds that target the CD24 signaling pathway, it is possible to identify potential therapeutics that could inhibit cancer progression. Moreover, this cell line can be utilized to understand the mechanism of action of novel anti-cancer drugs, facilitating the development of targeted therapies.

(2) Immune Response Modulation:

CD24 is also involved in the regulation of the immune response, acting as a checkpoint in both innate and adaptive immunity. The Human CD24 Stable Cell Line - CHO-K1 can be used to explore how CD24 signaling affects immune cell activation, differentiation, and the elimination of pathogens or tumor cells. This research can lead to the development of new strategies for immunotherapy, aiming to enhance the immune system's ability to fight cancer or to suppress it in the case of autoimmune diseases. Furthermore, studying the interaction between CD24-expressing cells and other components of the immune system can provide insights into the development of vaccines and novel immunomodulatory drugs.

(3) Stem Cell Research and Regenerative Medicine:

Given its role in cell adhesion and differentiation, CD24 expression is a critical factor in stem cell biology. The Human CD24 Stable Cell Line - CHO-K1 can serve as a tool to study the mechanisms underlying stem cell differentiation and the development of specific cell lineages. Understanding these processes is essential for advancing regenerative medicine, including tissue engineering and the development of stem cell therapies for degenerative diseases. Additionally, investigating CD24's involvement in these processes can uncover new targets for enhancing the efficiency of stem cell differentiation and integration into host tissues, paving the way for improved treatment options for a variety of conditions.

Customer Q&As

What is the specific methodology employed in generating the stable cell line for this product?

A: To generate the Human CD24 Stable Cell Line - CHO-K1, the process typically involves transfecting CHO-K1 cells with a plasmid containing the human CD24 gene along with a selectable marker gene, such as antibiotic resistance. Following transfection, cells are subjected to selective pressure using the appropriate antibiotic to allow only those cells containing the desired gene to survive. These surviving cells are then expanded and characterized to establish a stable cell line expressing human CD24.

What are the critical quality control measures employed during the establishment of this cell line?

A: During the establishment of the Human CD24 Stable Cell Line - CHO-K1, critical quality control measures are implemented at various stages. These measures may include verifying the expression of the CD24 gene through molecular techniques like PCR or Western blotting, confirming stable integration of the gene into the cell genome, assessing cell viability and growth characteristics, checking for genetic stability over passages, and ensuring consistent expression levels of CD24 over time.

What are the specific advantages of using CHO-K1 cells as the host for this stable cell line?

A: CHO-K1 cells offer several advantages as hosts for stable cell line development. They have a well-characterized genome and are easily transfected, allowing for efficient gene delivery and stable integration. Additionally, CHO cells are widely used in biopharmaceutical production due to their capacity for high-density growth in suspension culture, robust protein expression machinery, and post-translational modification capabilities, which are crucial for producing biotherapeutic proteins like CD24.

How is the stability of CD24 expression maintained in this cell line over multiple passages?

A: The stability of CD24 expression in the Human CD24 Stable Cell Line - CHO-K1 is maintained through stringent culture conditions and regular monitoring of cell characteristics. Cells are cultured under consistent conditions with proper nutrient supply and controlled environment to minimize variations. Additionally, regular subculturing and monitoring for any changes in growth rate, morphology, or expression levels are conducted. Any deviations from the expected behavior are thoroughly investigated, and corrective actions, such as readjustment of culture conditions or gene editing if necessary, are implemented to ensure stable CD24 expression over passages.

Could you provide information on the promoter region used to drive CD24 expression in this stable cell line?

A: The promoter region used to drive CD24 expression in the Human CD24 Stable Cell Line - CHO-K1 depends on the specific design of the expression vector. Commonly used promoters in CHO cell expression systems include the CMV (cytomegalovirus) promoter, SV40 (simian virus 40) promoter, and hybrid promoters such as the EF1α (elongation factor 1 alpha) promoter. These promoters drive robust and constitutive expression of the CD24 gene in CHO-K1 cells, ensuring sustained protein production.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

High specificity expression

The Human CD24 Stable Cell Line - CHO-K1, through stable transfection specific to the human CD24 gene, ensures high-level and specific expression of the CD24 protein, providing an important tool for studying the role of CD24 in cell signaling and immune regulation.

Germany

2020-10-25

Facilitates drug screening

Since the CD24 expressed by the Human CD24 Stable Cell Line - CHO-K1 is a key factor in the progression of various cancers and immune escape, this cell line can be used for high-throughput drug screening, accelerating the discovery of anticancer drugs.

United States

2023-01-09

Enhances biomarker research

The Human CD24 Stable Cell Line - CHO-K1 provides a stable platform for studying the expression changes of CD24 as a biomarker in different disease states, supporting early diagnosis and treatment monitoring.

Canada

2021-07-08

Improves experimental reproducibility

Because the Human CD24 Stable Cell Line - CHO-K1 provides consistent CD24 expression levels across multiple experimental batches, it enhances the reproducibility and reliability of experiments, ensuring the accuracy of data.

United States

2023-06-26

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RO0112	Human CD274 Stable Cell Line-HEK293T	Inquiry
CSC-RO0113	Human PDCD1LG2 Stable Cell Line-HEK293T	Inquiry
CSC-RO0080	Human CD28 Stable Cell Line-HEK293T	Inquiry
CSC-RO0002	Human CD276 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0013	Human HAVCR2 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0045	Human CD47 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0065	Mouse 4-1BB Stable Cell Line-CHO-K1	Inquiry
CSC-RO0100	Human CTLA4 Stable Cell Line-HEK293T	Inquiry
CSC-RO0012	Human CD274 Stable Cell Line-HEK293T	Inquiry
CSC-RO0011	Human CD274 Stable Cell Line-CHO-K1	Inquiry

Human CD24 Stable Cell Line - CHO-K1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the specific methodology employed in generating the stable cell line for this product?

What are the critical quality control measures employed during the establishment of this cell line?

What are the specific advantages of using CHO-K1 cells as the host for this stable cell line?

How is the stability of CD24 expression maintained in this cell line over multiple passages?

Could you provide information on the promoter region used to drive CD24 expression in this stable cell line?

High specificity expression

Facilitates drug screening

Enhances biomarker research

Improves experimental reproducibility

Related Products

Related Services