Human CD24 Stable Cell Line - HEK293T

Name: Human CD24 Stable Cell Line - HEK293T
SKU: CSC-RO0581
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0581

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0581
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human CD24.
Target Gene	CD24
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD24 CD24 molecule [ Homo sapiens ]
Gene Symbol	CD24
Synonyms	CD24A
Gene Description	CD24 molecule
Gene ID	100133941
Uni Prot ID	B6EC88
m RNA Refseq	NM_013230.2
Protein Refseq	NP_037362.1
Chromosome Location	6q21
Function	protein binding; protein kinase binding; protein tyrosine kinase activator activity; signal transducer activity;
Pathway	Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem;
MIM	600074

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Background

Case Study

Applications

Q & A

Customer Reviews

Stable human CD24 cell line A cell line called HEK293T has been modified to express CD24, a sialoglycoprotein that influences growth and differentiation signals and is present on mature B cells and granulocytes. On chromosome 6, the CD24 gene produces a precursor protein that is cleaved into a mature peptide with 32 amino acids that is attached to the cell surface via a glycosyl phosphatidylinositol (GPI) link. There are non-transcribed pseudogenes on chromosomes 1, 15, 20, and Y. Several transcript variants are produced by alternative splicing.

A protein-coding gene called CD24 has been linked to illnesses like multiple sclerosis and erythroleukemia. L1CAM interactions and nervous system development are two of its paths. From a functional standpoint, CD24 might be essential for cell type differentiation. Selectin-like ligand binding to CD24 carbohydrates, which is transduced by second messengers from the GPI-anchor, can trigger signaling. By regulating B-cell activation responses, it encourages proliferation that is dependent on AG while impeding terminal differentiation into cells that generate antibodies. Furthermore, CD24 may help regulate autoimmunity by selectively suppressing the immune response to danger-associated molecular patterns (DAMPs) in conjunction with SIGLEC10.

The researchers examined angiogenin, a ribonuclease linked to glioblastoma growth. Angiogenin upregulates CD24, a surface protein linked to glioblastoma's invasive development and poor prognosis. Increased CD24 expression helps glioblastoma cells associate with endothelial cells, allowing tumor invasion and nutrition availability via vasculature. This study emphasizes the importance of targeting the angiogenin-CD24 axis for glioblastoma treatment.

Figure 1 shows IHC staining of CD24 in WT and Ang1-/- mouse GBM tissues, with CD24-positive cells counted among 500 total cells. Figure 1. The researchers overexpressed CD24 in U87-GFP cells and co-cultured them with HUVEC cells to analyze endothelial association, cell circularity, and invasion in mouse brain tissues. (Yang H, et al., 2022)

Creative Biogene's Human CD24 Stable Cell Line - HEK293T provides a valuable tool for researchers investigating the mechanisms of tumor invasion and vascular association. This cell line can be utilized in studies to evaluate the therapeutic potential of CD24 targeting in various cancer contexts, offering insights into the pathways regulating cell adhesion and invasion.

1. Modification of CD24 Expression: The protein CD24, which is normally present on mature B cells and granulocytes and is involved in growth and differentiation signals, has been designed to be expressed on the HEK293T cell line.

2. Genetic Influence: A precursor protein that is cleaved into a mature peptide with 32 amino acids is encoded by the CD24 gene, which is found on chromosome 6. Its varied roles are facilitated by variants generated through alternative splicing.

3. Disease Association: The protein-coding gene CD24 is linked to illnesses such as erythroleukemia and multiple sclerosis, which affect processes including L1CAM interactions and nervous system development.

4. Membrane Activities: Functionally, CD24 controls B-cell activation responses and is involved in cell type differentiation. It affects processes of proliferation and differentiation by interactions with selectin-like ligands that set off signaling pathways.

5. Immunomodulation: CD24, maybe in conjunction with SIGLEC10, may also regulate autoimmunity by limiting immunological responses to danger-associated molecular patterns (DAMPs).

Customer Q&As

What is the expression level of this human CD24 stable cell line? Is there relevant quality control data?

A: Our human CD24 stable cell lines express stable levels and undergo strict quality control. We regularly validate cell lines and record cell line expression level data. Customers can request this data from us to understand the expression levels of our products and their stability.

How functional is this stable cell line when studying the role of CD24 in cell adhesion and signaling? Is relevant functional verification data provided?

A: Our human CD24 stable cell lines are important in cell adhesion and signaling studies and are fully functional. We provide detailed functional verification data, including cell adhesion experiments, signaling pathway analysis, etc. Customers can refer to these data to evaluate the performance of our products.

Is this human CD24 stable cell line suitable for drug screening experiments? Is there relevant literature support?

A: Our human CD24 stable cell lines have been widely used in drug screening experiments. We provide relevant literature support demonstrating the potential of our products in drug screening. Customers can refer to these documents to understand the performance and application range of our products.

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Customer Reviews

Efficient

In my experiments, the cell morphology was good and there were no abnormal changes, ensuring the accuracy of the data. HEK293T cells have powerful gene manipulation capabilities and protein expression capabilities, making them an ideal tool for biomedical research. The cell products provided by the supplier are of excellent quality and the overall experience is very professional.

Canada

2020-04-24

Satisfy

The customer service staff are very professional, answered my questions in detail, and provided all-round technical support. The cells grew well during the culture process without any contamination issues, which made the experiment go very smoothly. The gene expression of CD24 is stable, ensuring the reliability of experimental data. The platform provides detailed instructions for use, making the entire use process very smooth.

French

2020-05-30

Outstanding

While conducting research using the Human CD24 Stable Cell Line - HEK293T, I found that this cell line is ideal for studying growth and differentiation signals in B cells and granulocytes. The cells grew rapidly in the culture medium and maintained good shape and vitality. This cell line has performed well in research applications and is an ideal tool for further research.

French

2023-12-02

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Human CD24 Stable Cell Line - HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the expression level of this human CD24 stable cell line? Is there relevant quality control data?

How functional is this stable cell line when studying the role of CD24 in cell adhesion and signaling? Is relevant functional verification data provided?

Is this human CD24 stable cell line suitable for drug screening experiments? Is there relevant literature support?

Efficient

Satisfy

Outstanding

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