Cat.No. : CSC-RO0581 Host Cell: HEK293T
| Cat. No. | CSC-RO0581 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human CD24. |
| Gene | CD24 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers examined angiogenin, a ribonuclease linked to glioblastoma growth. Angiogenin upregulates CD24, a surface protein linked to glioblastoma's invasive development and poor prognosis. Increased CD24 expression helps glioblastoma cells associate with endothelial cells, allowing tumor invasion and nutrition availability via vasculature. This study emphasizes the importance of targeting the angiogenin-CD24 axis for glioblastoma treatment.
Figure 1. The researchers overexpressed CD24 in U87-GFP cells and co-cultured them with HUVEC cells to analyze endothelial association, cell circularity, and invasion in mouse brain tissues. (Yang H, et al., 2022)
Creative Biogene's Human CD24 Stable Cell Line - HEK293T provides a valuable tool for researchers investigating the mechanisms of tumor invasion and vascular association. This cell line can be utilized in studies to evaluate the therapeutic potential of CD24 targeting in various cancer contexts, offering insights into the pathways regulating cell adhesion and invasion.
A: Our human CD24 stable cell lines express stable levels and undergo strict quality control. We regularly validate cell lines and record cell line expression level data. Customers can request this data from us to understand the expression levels of our products and their stability.
A: Our human CD24 stable cell lines are important in cell adhesion and signaling studies and are fully functional. We provide detailed functional verification data, including cell adhesion experiments, signaling pathway analysis, etc. Customers can refer to these data to evaluate the performance of our products.
A: Our human CD24 stable cell lines have been widely used in drug screening experiments. We provide relevant literature support demonstrating the potential of our products in drug screening. Customers can refer to these documents to understand the performance and application range of our products.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
In my experiments, the cell morphology was good and there were no abnormal changes, ensuring the accuracy of the data. HEK293T cells have powerful gene manipulation capabilities and protein expression capabilities, making them an ideal tool for biomedical research. The cell products provided by the supplier are of excellent quality and the overall experience is very professional.
The customer service staff are very professional, answered my questions in detail, and provided all-round technical support. The cells grew well during the culture process without any contamination issues, which made the experiment go very smoothly. The gene expression of CD24 is stable, ensuring the reliability of experimental data. The platform provides detailed instructions for use, making the entire use process very smooth.
While conducting research using the Human CD24 Stable Cell Line - HEK293T, I found that this cell line is ideal for studying growth and differentiation signals in B cells and granulocytes. The cells grew rapidly in the culture medium and maintained good shape and vitality. This cell line has performed well in research applications and is an ideal tool for further research.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
