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Cat.No. : CSC-RO1029 Host Cell: HEK293T
| Cat. No. | CSC-RO1029 |
| Description | HEK293-CD24 cell line is engineered to stably overexpress human CD24. |
| Gene | CD24 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The stable expression of CD24 protein is achieved by introducing the CD24 gene into HEK293 cells using methods such as transfection or viral infection, allowing the cells to stably express CD24 protein.
A: This cell line can be used to investigate the role of CD24 in immune cell recognition, signal transduction, and immune responses, thereby revealing the function of CD24 in immune responses.
A: The cell growth characteristics can be evaluated by observing cell morphology, growth rate, cell density, and doubling time. In addition, quantitative assessment of cell viability can be performed using experimental methods such as MTT or CCK-8.
A: This cell line can be used to screen and evaluate drugs targeting CD24-related pathways, such as assessing the potential efficacy and safety of drugs by detecting changes in CD24 expression or function after drug treatment.
A: To avoid cell contamination, experiments should be conducted under aseptic conditions, regularly check the cleanliness of the cell culture environment, and use sterile reagents and consumables. At the same time, strict aseptic operation training should be provided for experimental personnel.
A: The cells can be stored in liquid nitrogen to maintain their stability. Before freezing, cells can be suspended in culture medium containing an appropriate concentration of cryoprotectant (such as DMSO) and gradually cooled in a programmable freezer. When thawing, quickly transfer the cells to a 37°C water bath and then add pre-warmed culture medium to reduce cell damage.
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