Human CD24 Stable Cell Line-HEK293T

Name: Human CD24 Stable Cell Line-HEK293T
SKU: CSC-RO1029
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1029

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1029
Description	HEK293-CD24 cell line is engineered to stably overexpress human CD24.
Target Gene	CD24
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD24 CD24 molecule [ Homo sapiens ]
Gene Symbol	CD24
Synonyms	CD24A
Gene Description	CD24 molecule
Gene ID	100133941
Uni Prot ID	B6EC88
m RNA Refseq	NM_013230.2
Protein Refseq	NP_037362.1
Chromosome Location	6q21
Function	protein binding; protein kinase binding; protein tyrosine kinase activator activity; signal transducer activity;
Pathway	Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem;
MIM	600074

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Q & A

Customer Reviews

Customer Q&As

How is the stable expression of CD24 protein achieved in the Human CD24 Stable Cell Line-HEK293?

A: The stable expression of CD24 protein is achieved by introducing the CD24 gene into HEK293 cells using methods such as transfection or viral infection, allowing the cells to stably express CD24 protein.

What is the role of Human CD24 Stable Cell Line-HEK293 in studying immune responses?

A: This cell line can be used to investigate the role of CD24 in immune cell recognition, signal transduction, and immune responses, thereby revealing the function of CD24 in immune responses.

How can the cell growth characteristics of Human CD24 Stable Cell Line-HEK293 be evaluated?

A: The cell growth characteristics can be evaluated by observing cell morphology, growth rate, cell density, and doubling time. In addition, quantitative assessment of cell viability can be performed using experimental methods such as MTT or CCK-8.

What are the applications of Human CD24 Stable Cell Line-HEK293 in drug screening?

A: This cell line can be used to screen and evaluate drugs targeting CD24-related pathways, such as assessing the potential efficacy and safety of drugs by detecting changes in CD24 expression or function after drug treatment.

How can cell contamination be avoided when using Human CD24 Stable Cell Line-HEK293 for experiments?

A: To avoid cell contamination, experiments should be conducted under aseptic conditions, regularly check the cleanliness of the cell culture environment, and use sterile reagents and consumables. At the same time, strict aseptic operation training should be provided for experimental personnel.

How can Human CD24 Stable Cell Line-HEK293 cells be stored to maintain their stability?

A: The cells can be stored in liquid nitrogen to maintain their stability. Before freezing, cells can be suspended in culture medium containing an appropriate concentration of cryoprotectant (such as DMSO) and gradually cooled in a programmable freezer. When thawing, quickly transfer the cells to a 37°C water bath and then add pre-warmed culture medium to reduce cell damage.

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