Human CCR7 Stable Cell Line-HEK293

Name: Human CCR7 Stable Cell Line-HEK293
SKU: CSC-RG1828
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1828

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1828
Description	HEK293-CCR7 cell line is engineered to stably overexpress human CCR7.
Target Gene	CCR7
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CCR7 C-C motif chemokine receptor 7 [ Homo sapiens (human) ]
Gene Symbol	CCR7
Synonyms	BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Gene ID	1236
Uni Prot ID	P32248
Chromosome Location	17q12-q21.2
Function	C-C chemokine receptor activity; C-C motif chemokine 19 receptor activity; C-C motif chemokine 21 receptor activity; G-protein coupled receptor activity; chemokine (C-C motif) ligand 19 binding; chemokine (C-C motif) ligand 21 binding;
Pathway	Chemokine receptors bind chemokines, organism-specific biosystem (from REACTOME); Chemokine signaling pathway, organism-specific biosystem (from KEGG); Chemokine signaling pathway, conserved biosystem (from KEGG); Class A/1 (Rhodopsin-like receptors), organism-specific biosystem (from REACTOME);Cytokine-cytokine receptor interaction, organism-specific biosystem (from KEGG); Cytokine-cytokine receptor interaction, conserved biosystem (from KEGG); G alpha (i) signalling events, organism-specific biosystem (from REACTOME);

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