Rat Tshr Stable Cell Line-HEK293T

Name: Rat Tshr Stable Cell Line-HEK293T
SKU: CSC-RG0271
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0271

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0271
Background	The thyroid stimulating hormone receptor (TSHR) is expressed in the membrane of thyroid follicular cells. It is involved in regulating thyrocyte cell growth and function. TSHR is also the target autoantigen in thyroid autoimmune diseases. Autoantibodies to TSHR that act as agonists are responsible for the hyperthyroidism of Graves" Disease. Another class of autoantibodies that block the binding of TSH to TSHR may mediate the hypothyroidism associated with Hashimoto"s thyroiditis, primary myxoedema, and neonatal hypothyroidism.
Target Gene	Tshr
Abbr	HEK293T-RatTshr
Alias	TSHR, TSH-R, TSHRA
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tshr thyroid stimulating hormone receptor [ Mus musculus ]
Gene Symbol	Tshr
Synonyms	hyt; pet; AI481368; hypothroid
Gene Description	thyroid stimulating hormone receptor
GeneID	22095
Uni ProtID	P47750
mRNA Refseq	NM_011648.5
Protein Refseq	NP_035778.3
Chromosome Location	12 D3; 12 37.0 cM
Function	G-protein coupled receptor activity; protein-hormone receptor activity; signal transducer activity; thyroid-stimulating hormone receptor activity; thyroid-stimulating hormone receptor activity;
Pathway	Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; Non-odorant GPCRs, organism-specific biosystem; Peptide GPCRs, organism-specific biosystem;

Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *