Rat Chrm4 Stable Cell Line-CHO-K1

Name: Rat Chrm4 Stable Cell Line-CHO-K1
SKU: CSC-RG0877
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0877

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0877
Target Gene	Chrm4
Abbr	CHO-RatChrm4
Alias	CHRM4, M4
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Chrm4 cholinergic receptor, muscarinic 4 [ Mus musculus ]
Gene Symbol	Chrm4
Synonyms	M4; Chrm-4
Gene Description	cholinergic receptor, muscarinic 4
Gene ID	12672
Uni Prot ID	P32211
m RNA Refseq	NM_007699.2
Protein Refseq	NP_031725.1
Chromosome Location	2 E1; 2 49.0 cM
Function	G-protein coupled acetylcholine receptor activity; G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity; signal transducer activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem;

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Muscarinic acetylcholine receptors are relatively abundant and mediate many of the diverse actions of acetylcholine in the CNS, as well as throughout non-nervous tissues innervated by the parasympathetic nervous system. There are five muscarinic receptor subtypes referred to as M1, M2, M3, M4 and M5 which belong to GPCR superfamily. The odd-numbered muscarinic receptors (M1, M3, M5) couple efficiently through Gq/11, to activate of phospholipase C, that initiates the phosphatidylinositol turnover response. The even-numbered muscarinic receptors (M2, M4) inhibit adenylyl caclase activity via activation of the Gi class of G proteins. M2 and M4 receptors also activate G protein-coupled potassium channel, which leads to hyperpolarization of the plasma membrane of excitable cells.

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