Human CHRM4 Stable Cell Line-CHO-K1

Name: Human CHRM4 Stable Cell Line-CHO-K1
SKU: CSC-RG0878
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0878

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0878
Background	The five muscarinic receptor subtypes all mediate the action of acetylcholine. The odd-numbered receptors (m1, m3, and m5) couple efficiently through Gq/11 to activate phospholipase C and lead to elevation of intracellular calcium, whereas the even-numbered receptors (m2, m4) are efficiently coupled to Gi and lead to inhibition of intracellular cAMP along with several other signal transduction pathways.
Target Gene	CHRM4
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCHRM4
Alias	CHRM4, HM4, M4R
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM4 cholinergic receptor, muscarinic 4 [ Homo sapiens ]
Gene Symbol	CHRM4
Synonyms	HM4; M4R
Gene Description	cholinergic receptor, muscarinic 4
Gene ID	1132
Uni Prot ID	P08173
m RNA Refseq	NM_000741.2
Protein Refseq	NP_000732.2
Chromosome Location	11p12-p11.2
Function	G-protein coupled acetylcholine receptor activity; guanyl-nucleotide exchange factor activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem;
MIM	118495

Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *