Cat.No. : CSC-RO0055 Host Cell: HEK293
| Cat. No. | CSC-RO0055 |
| Description | This cell line is a stably transfected cell line which expresses human Toll-like receptor 3 (TLR3) with an N-terminal HA tag. |
| Gene | TLR3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line aids in the development and testing of new therapeutic drugs, especially those targeting TLR3 or the NF-κB pathway. Its use in high-throughput screening of drug candidates accelerates the discovery and validation of novel therapeutics.
A: Limitations include the risk of not accurately representing in vivo conditions due to being a transformed cell line, potential clonal variability, and the presence of viral DNA fragments in the genome. These factors can affect the generalizability of research findings.
A: In immunology, this cell line is used to study the innate immune response, particularly the mechanisms and roles of TLR3 in antiviral responses. It helps in understanding how TLR3 recognizes viral double-stranded RNA and triggers immune signaling.
A: Researchers should consider the cell line's specific response to TLR3 agonists, its utility in studying NF-κB signaling pathways, and its role in drug discovery and development. It's also important to acknowledge the limitations and variability that can arise from using a transformed cell line in research.
A: The HEK293 cell lines, including the TLR3 variant, are designated as Biosafety Level 2. It is advised to assume that all cultures may harbor latent viruses or uncharacterized organisms. Safety precautions like using pipette aids, minimizing aerosols, and avoiding eating or drinking near the cultures are recommended.
A: Currently, there is limited information available on specific regulatory and ethical considerations for this cell line. However, like other cell lines, it's essential to consider the source of the cells, consent for use, and ethical guidelines for handling human-derived cell lines in research.
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