Human TLR3 Stable Cell Line-HEK293

Name: Human TLR3 Stable Cell Line-HEK293
SKU: CSC-RO0055
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0055

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0055
Description	This cell line is a stably transfected cell line which expresses human Toll-like receptor 3 (TLR3) with an N-terminal HA tag.
Target Gene	TLR3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Source	HEK293
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TLR3 toll like receptor 3 [ Homo sapiens (human) ]
Gene Symbol	TLR3
Synonyms	CD283; IIAE2
GeneID	7098
Uni ProtID	O15455
Chromosome Location	4q35.1

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Background

Q & A

Customer Reviews

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses.

Customer Q&As

How does the Human TLR3 Stable Cell Line-HEK293 contribute to advancements in biopharmaceuticals?

A: This cell line aids in the development and testing of new therapeutic drugs, especially those targeting TLR3 or the NF-κB pathway. Its use in high-throughput screening of drug candidates accelerates the discovery and validation of novel therapeutics.

What are the limitations of using the Human TLR3 Stable Cell Line-HEK293 in research?

A: Limitations include the risk of not accurately representing in vivo conditions due to being a transformed cell line, potential clonal variability, and the presence of viral DNA fragments in the genome. These factors can affect the generalizability of research findings.

How is the Human TLR3 Stable Cell Line-HEK293 typically used in immunological research?

A: In immunology, this cell line is used to study the innate immune response, particularly the mechanisms and roles of TLR3 in antiviral responses. It helps in understanding how TLR3 recognizes viral double-stranded RNA and triggers immune signaling.

What are some unique scientific research considerations when working with the Human TLR3 Stable Cell Line-HEK293?

A: Researchers should consider the cell line's specific response to TLR3 agonists, its utility in studying NF-κB signaling pathways, and its role in drug discovery and development. It's also important to acknowledge the limitations and variability that can arise from using a transformed cell line in research.

What are the biosafety considerations when handling the Human TLR3 Stable Cell Line-HEK293?

A: The HEK293 cell lines, including the TLR3 variant, are designated as Biosafety Level 2. It is advised to assume that all cultures may harbor latent viruses or uncharacterized organisms. Safety precautions like using pipette aids, minimizing aerosols, and avoiding eating or drinking near the cultures are recommended.

What are the regulatory and ethical considerations associated with using the Human TLR3 Stable Cell Line-HEK293?

A: Currently, there is limited information available on specific regulatory and ethical considerations for this cell line. However, like other cell lines, it's essential to consider the source of the cells, consent for use, and ethical guidelines for handling human-derived cell lines in research.

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