Cat.No. : CSC-RG0054 Host Cell: HEK293
| Cat. No. | CSC-RG0054 |
| Background | This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5"-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neuropeptide substance K, also referred to as neurokinin A. |
| Gene | TACR2 |
| Gene Species | Homo sapiens (Human) |
| Alias | TACR2, NKNAR, TAC2R, NK2R, SKR, NK-2R |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TACR2-SNAP Stable Cell Line-HEK293 serves as a valuable model system for studying the role of TACR2 (T cell activation receptor 2) in T cell activation. By stably expressing TACR2 with a SNAP tag, researchers can visualize and manipulate the protein in real-time, providing insights into its function, signaling pathways, and potential therapeutic targets in immune responses.
A: The Human TACR2-SNAP Stable Cell Line-HEK293 can be employed in high-throughput screening assays to identify compounds that modulate TACR2 activity. By monitoring changes in TACR2 localization or function upon compound exposure, researchers can identify potential drug candidates that affect T cell activation and immune responses.
A: The Human TACR2-SNAP Stable Cell Line-HEK293 allows for the site-specific attachment of biotin or other affinity tags to TACR2, enabling the detection and analysis of post-translational modifications such as phosphorylation, ubiquitination, or glycosylation. This can provide valuable information on the regulation of TACR2 function and its role in cellular processes.
A: The Human TACR2-SNAP Stable Cell Line-HEK293 can be used to study the effects of patient-specific genetic variations on TACR2 function. This information can help in the development of personalized medicine strategies, such as tailored T cell therapies, by providing insights into how individual genetic backgrounds may influence the efficacy and safety of these treatments.
A: The Human TACR2-SNAP Stable Cell Line-HEK293 can be utilized in studies aimed at understanding the dysregulation of TACR2 in autoimmune diseases. By comparing the behavior of TACR2-SNAP in healthy cells versus those derived from patients with autoimmune conditions, researchers can identify potential molecular mechanisms underlying disease pathogenesis and develop targeted therapies.
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