Human TACR2-SNAP Stable Cell Line-HEK293

Name: Human TACR2-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0054
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0054

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0054
Background	This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5"-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neuropeptide substance K, also referred to as neurokinin A.
Target Gene	TACR2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuTACR2-SNAP
Alias	TACR2, NKNAR, TAC2R, NK2R, SKR, NK-2R
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TACR2 tachykinin receptor 2 [ Homo sapiens ]
Gene Symbol	TACR2
Synonyms	SKR; NK2R; NKNAR; TAC2R
Gene Description	tachykinin receptor 2
GeneID	6865
Uni ProtID	P21452
mRNA Refseq	NM_001057.2
Protein Refseq	NP_001048.2
Chromosome Location	10q22.1
Function	tachykinin receptor activity;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem;
MIM	162321

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Customer Reviews

Customer Q&As

How does the Human TACR2-SNAP Stable Cell Line-HEK293 model contribute to the understanding of T cell activation?

A: The Human TACR2-SNAP Stable Cell Line-HEK293 serves as a valuable model system for studying the role of TACR2 (T cell activation receptor 2) in T cell activation. By stably expressing TACR2 with a SNAP tag, researchers can visualize and manipulate the protein in real-time, providing insights into its function, signaling pathways, and potential therapeutic targets in immune responses.

How can the Human TACR2-SNAP Stable Cell Line-HEK293 be utilized in drug screening for immunomodulatory compounds?

A: The Human TACR2-SNAP Stable Cell Line-HEK293 can be employed in high-throughput screening assays to identify compounds that modulate TACR2 activity. By monitoring changes in TACR2 localization or function upon compound exposure, researchers can identify potential drug candidates that affect T cell activation and immune responses.

How does the Human TACR2-SNAP Stable Cell Line-HEK293 aid in the study of TACR2 post-translational modifications?

A: The Human TACR2-SNAP Stable Cell Line-HEK293 allows for the site-specific attachment of biotin or other affinity tags to TACR2, enabling the detection and analysis of post-translational modifications such as phosphorylation, ubiquitination, or glycosylation. This can provide valuable information on the regulation of TACR2 function and its role in cellular processes.

How does the Human TACR2-SNAP Stable Cell Line-HEK293 contribute to the development of personalized medicine in the context of T cell therapies?

A: The Human TACR2-SNAP Stable Cell Line-HEK293 can be used to study the effects of patient-specific genetic variations on TACR2 function. This information can help in the development of personalized medicine strategies, such as tailored T cell therapies, by providing insights into how individual genetic backgrounds may influence the efficacy and safety of these treatments.

How can the Human TACR2-SNAP Stable Cell Line-HEK293 be used to investigate the role of TACR2 in autoimmune diseases?

A: The Human TACR2-SNAP Stable Cell Line-HEK293 can be utilized in studies aimed at understanding the dysregulation of TACR2 in autoimmune diseases. By comparing the behavior of TACR2-SNAP in healthy cells versus those derived from patients with autoimmune conditions, researchers can identify potential molecular mechanisms underlying disease pathogenesis and develop targeted therapies.

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