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Human S1PR1 Stable Cell Line-CHO-K1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RG0834

Host Cell :   CHO-K1 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RG0834
Background Edg receptors constitute of family of 8 GPCRs (designated as Edg-1 through Edg-8) that can be further sub-classified as sphingosine 1-phosphate receptors (S-1-P) and lysophosphatidic acid (LPA) receptors. The Edg-1 receptor is an S-1-P receptor that is coupled to a variety of intracellular responses via Gi and Go. The Edg-1 receptor was initially cloned as an immediate-early gene induced during differentiation of human endothelial cells, an in vitro model of angiogenesis. It has been shown to play a critical role in regulating cellular proliferation and survival.
Target Gene S1PR1
Gene Species Homo sapiens (Human)
Abbr CHO-HuS1PR1
Alias S1PR1, EDG1, CD363, D1S3362, edg 1, S1P1, ECGF1, EDG-1, CHEDG1, FLJ58121
Host Cell CHO-K1
Host Cell Species Cricetulus griseus (Chinese hamster)
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Research on the mechanisms of GPCR-related diseases

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Morphology Epithelial-like
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name S1PR1 sphingosine-1-phosphate receptor 1 [ Homo sapiens ]
Gene Symbol S1PR1
Synonyms S1PR1; sphingosine-1-phosphate receptor 1; EDG1, endothelial differentiation, sphingolipid G protein coupled receptor, 1; sphingosine 1-phosphate receptor 1; CD363; D1S3362; edg 1; S1P receptor 1; S1P receptor Edg-1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; EDG1; S1P1; ECGF1; EDG-1; CHEDG1; FLJ58121;
GeneID 1901
Uni ProtID P21453
mRNA Refseq BC018650
Chromosome Location 1p21
Function G-protein coupled rec
Pathway Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Lysosphingolipid and LPA receptors, organism-specific biosystem;
MIM 601974
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High Expression and Great Viability

Creative Biogene's S1PR1 cell line arrived with excellent viability and recovered quickly upon thawing. We verified the surface expression via flow cytometry, and it far exceeded our expectations. The cells are robust and incredibly easy to maintain in culture.

United States

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