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Cat.No. : CSC-RG0027 Host Cell: Hep G2
| Cat. No. | CSC-RG0027 |
| Background | This gene encodes S1P1 receptor which is a member of the G protein-coupled receptor superfamily (S1P1-5) originally known as EDG receptors. Through the interaction with this receptor family, sphingosine-1-phosphate stimulates diverse cellular responses, including cytoskeletal changes, proliferation and migration. The S1P1R is widely expressed including on endothelial cells. The physiological effects of S1P1R have been described in immune and vascular systems |
| Gene | S1PR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | S1PR1, EDG1, CD363, D1S3362, edg 1, EDG1, S1P1, ECGF1, EDG-1, CHEDG1 |
| Host Cell | Hep G2 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human S1PR1-tGFP Stable Cell Line-HepG2 is crucial for investigating the function of the S1PR1 (Sphingosine-1-phosphate receptor 1) in cellular processes. By incorporating a green fluorescent protein (tGFP) tag, researchers can visualize and track the localization and dynamics of S1PR1 within the HepG2 cells, which are a line of liver cancer cells. This allows for a better understanding of how S1PR1 contributes to cell signaling, particularly in liver physiology and pathology.
A: The HepG2 cell line, when stably expressing S1PR1 with a tGFP tag, provides a model system to screen for compounds that modulate S1PR1 activity. This can lead to the discovery of new therapeutic agents that target S1PR1, potentially offering targeted treatments for liver diseases where S1PR1 signaling is dysregulated.
A: Establishing a stable cell line involves the integration of the S1PR1-tGFP construct into the host cell genome, which can be challenging due to the random nature of integration. Overcoming these challenges often involves using viral vectors for efficient transduction, followed by selection pressure to isolate cells with stable integration and expression of the construct.
A: The fluorescent tag allows researchers to monitor S1PR1's subcellular localization during cell migration and invasion, which are critical processes in cancer progression. By observing the dynamics of S1PR1-tGFP, insights can be gained into how S1PR1 signaling influences these processes in HepG2 cells.
A: The presence of tGFP allows for the use of fluorescence resonance energy transfer (FRET) or co-immunoprecipitation (co-IP) assays to study protein-protein interactions involving S1PR1. This can help elucidate the molecular mechanisms of S1PR1 signaling in HepG2 cells.
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