Human S1PR1-tGFP Stable Cell Line-HepG2

Name: Human S1PR1-tGFP Stable Cell Line-HepG2
SKU: CSC-RG0027
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0027

Host Cell : HepG2 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0027
Background	This gene encodes S1P1 receptor which is a member of the G protein-coupled receptor superfamily (S1P1-5) originally known as EDG receptors. Through the interaction with this receptor family, sphingosine-1-phosphate stimulates diverse cellular responses, including cytoskeletal changes, proliferation and migration. The S1P1R is widely expressed including on endothelial cells. The physiological effects of S1P1R have been described in immune and vascular systems
Target Gene	S1PR1
Gene Species	Homo sapiens (Human)
Abbr	HepG2-HuS1PR1-tGFP
Alias	S1PR1, EDG1, CD363, D1S3362, edg 1, EDG1, S1P1, ECGF1, EDG-1, CHEDG1
Host Cell	HepG2
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	S1PR1 sphingosine-1-phosphate receptor 1 [ Homo sapiens ]
Gene Symbol	S1PR1
Synonyms	S1PR1; sphingosine-1-phosphate receptor 1; EDG1, endothelial differentiation, sphingolipid G protein coupled receptor, 1; sphingosine 1-phosphate receptor 1; CD363; D1S3362; edg 1; S1P receptor 1; S1P receptor Edg-1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; EDG1; S1P1; ECGF1; EDG-1; CHEDG1; FLJ58121;
GeneID	1901
Uni ProtID	P21453
mRNA Refseq	BC018650
Chromosome Location	1p21
Function	G-protein coupled rec
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Lysosphingolipid and LPA receptors, organism-specific biosystem;
MIM	601974

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Customer Q&As

What is the significance of the Human S1PR1-tGFP Stable Cell Line-HepG2 in studying the role of S1PR1 in cell signaling pathways?

A: The Human S1PR1-tGFP Stable Cell Line-HepG2 is crucial for investigating the function of the S1PR1 (Sphingosine-1-phosphate receptor 1) in cellular processes. By incorporating a green fluorescent protein (tGFP) tag, researchers can visualize and track the localization and dynamics of S1PR1 within the HepG2 cells, which are a line of liver cancer cells. This allows for a better understanding of how S1PR1 contributes to cell signaling, particularly in liver physiology and pathology.

How does the Human S1PR1-tGFP Stable Cell Line-HepG2 aid in the development of targeted therapies for liver diseases?

A: The HepG2 cell line, when stably expressing S1PR1 with a tGFP tag, provides a model system to screen for compounds that modulate S1PR1 activity. This can lead to the discovery of new therapeutic agents that target S1PR1, potentially offering targeted treatments for liver diseases where S1PR1 signaling is dysregulated.

What are the technical challenges associated with establishing a Human S1PR1-tGFP Stable Cell Line-HepG2, and how are they typically overcome?

A: Establishing a stable cell line involves the integration of the S1PR1-tGFP construct into the host cell genome, which can be challenging due to the random nature of integration. Overcoming these challenges often involves using viral vectors for efficient transduction, followed by selection pressure to isolate cells with stable integration and expression of the construct.

How does the Human S1PR1-tGFP Stable Cell Line-HepG2 contribute to the understanding of S1PR1's role in cell migration and invasion?

A: The fluorescent tag allows researchers to monitor S1PR1's subcellular localization during cell migration and invasion, which are critical processes in cancer progression. By observing the dynamics of S1PR1-tGFP, insights can be gained into how S1PR1 signaling influences these processes in HepG2 cells.

How does the Human S1PR1-tGFP Stable Cell Line-HepG2 facilitate the study of S1PR1's interaction with other cellular proteins?

A: The presence of tGFP allows for the use of fluorescence resonance energy transfer (FRET) or co-immunoprecipitation (co-IP) assays to study protein-protein interactions involving S1PR1. This can help elucidate the molecular mechanisms of S1PR1 signaling in HepG2 cells.

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