Human KCNQ2/KCNQ3 Stable Cell Line-HEK293

Name: Human KCNQ2/KCNQ3 Stable Cell Line-HEK293
SKU: CSC-RI0145
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0145

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0145
Description	This cell line is engineered to overexpress human KCNQ3.
Background	The human KCNQ2 and KCNQ3 genes encode the pore-forming subunits of Kv7.2 and Kv7.3, respectively. These subunits form heteromeric voltage-gated potassium channels that underlie M-current that regulates repetitive neuronal activity. Mutations in KCNQ2 or KCNQ3 that reduce M-current amplitude are responsible for some forms of benign familial neonatal seizure. Kv7.2/Kv7.3 channels are therapeutic targets in seizure, stroke, migraine, dementia, bipolar disorder, anxiety, and neuropathic pain.
Target Gene	KCNQ3
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNQ2/KCNQ3
Alias	KCNQ3, EBN2, Kv7.3, BFNC2, KV7.3, FLJ37386, FLJ38392, DKFZp686C0248
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNQ3 potassium voltage-gated channel, KQT-like subfamily, member 3 [ Homo sapiens ]
Gene Symbol	KCNQ3
Synonyms	EBN2; BFNC2; KV7.3
GeneID	3786
Uni ProtID	B4DJY4
mRNA Refseq	NM_001204824.1
Protein Refseq	NP_001191753.1
Chromosome Location	8q24
Function	potassium channel activity; voltage-gated potassium channel activity;
Pathway	Axon guidance, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	602232

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