Human KCNJ3/KCNJ5 Stable Cell Line-HEK293

Name: Human KCNJ3/KCNJ5 Stable Cell Line-HEK293
SKU: CSC-RI0119
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0119

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0119
Description	This cell line is engineered to overexpress human KCNJ5.
Background	The human KCNJ3 and KCNJ5 genes encode the pore-forming subunits of heteromeric Kir3.1/Kir3.4, an inward rectifier potassium channel. Kir3.1/Kir3.4 channels expressed in heart are linked to M2 muscarinic acetylcholine receptors that mediate vagal inhibitory control of heart rate. Kir3.1/Kir3.4 is a potential target for treatment of atrial fibrillation.
Target Gene	KCNJ5
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNJ3/KCNJ5
Alias	KCNJ5, CIR, GIRK4, KATP1, Kir3.4, IRK-4, LQT13, KIR3.4
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNJ5 potassium inwardly-rectifying channel, subfamily J, member 5 [ Homo sapiens ]
Gene Symbol	KCNJ5
Synonyms	CIR; GIRK4; KATP1; LQT13; KIR3.4
Gene Description	potassium inwardly-rectifying channel, subfamily J, member 5
Gene ID	3762
Uni Prot ID	P48544
m RNA Refseq	NM_000890.3
Protein Refseq	NP_000881.3
Chromosome Location	11q24
Function	G-protein activated inward rectifier potassium channel activity; contributes_to inward rectifier potassium channel activity; protein binding;
Pathway	Activation of G protein gated Potassium channels, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; G protein gated Potassium channels, organism-specific biosystem; GABA B receptor activation, organism-specific biosystem;
MIM	600734

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