Human KCNJ1 Stable Cell Line-HEK293

Name: Human KCNJ1 Stable Cell Line-HEK293
SKU: CSC-RI0114
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0114

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0114
Description	This cell line is engineered to overexpress human KCNJ1.
Background	Kir1.1 is an inwardly rectifying potassium channel expessed in kidney, skeletal muscle, liver, pancreas, spleen, and the central nervous system. Mutations in Kir1.1 are responsible for Bartter"s syndrome (renal salt loss) and hereditary hypertension with hyperkalemia. Kir1.1 has therapeutic potential in treatment of hypertension.
Target Gene	KCNJ1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNJ1
Alias	KCNJ1, Kir1.1, ROMK1, ROMK, KIR1.1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNJ1 potassium inwardly-rectifying channel, subfamily J, member 1 [ Homo sapiens ]
Gene Symbol	KCNJ1
Synonyms	ROMK; ROMK1; KIR1.1
Gene Description	potassium inwardly-rectifying channel, subfamily J, member 1
Gene ID	3758
Uni Prot ID	P48048
m RNA Refseq	NM_153764.1
Protein Refseq	NP_722448.1
Chromosome Location	11q24
Function	ATP binding; inward rectifier potassium channel activity;
Pathway	Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Inwardly rectifying K+ channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	600359

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Q & A

Customer Reviews

Customer Q&As

What are the applications of the Human KCNJ1 Stable Cell Line-HEK293 in cardiology research?

A: It can be used to study the role of KCNJ1 in cardiac cells, particularly in understanding the molecular mechanisms of arrhythmias and heart failure.

How to ensure the accuracy and reproducibility of gene expression studies using this cell line?

A: Ensure data accuracy and reproducibility through standardized experimental procedures and conditions, as well as by conducting multiple replicates.

What are the potential uses of the Human KCNJ1 Stable Cell Line-HEK293 in neurological disease model research?

A: As KCNJ1 also functions in the nervous system, this cell line can be used to model and study neurological diseases related to potassium channels.

What drug targets should be considered when using the Human KCNJ1 Stable Cell Line-HEK293 for drug screening?

A: Drugs that may affect the activity of the KCNJ1 channel, including those that regulate potassium ion permeability and potential, should be considered.

What are the specific applications of this cell line in renal pathology research?

A: Since KCNJ1 plays a significant role in regulating potassium balance in the kidneys, this cell line is particularly suitable for studying renal diseases such as Bartter syndrome.

How was the Human KCNJ1 Stable Cell Line-HEK293 constructed using gene-editing technology?

A: The cell line was constructed by stably integrating the human KCNJ1 gene (potassium channel subfamily J member 1) into the genome of HEK293 cells using gene-editing technologies like CRISPR/Cas9.

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