Human KCNA2 Stable Cell Line-HEK293

Name: Human KCNA2 Stable Cell Line-HEK293
SKU: CSC-RI0123
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0123

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0123
Description	This cell line is engineered to overexpress human KCNA2.
Background	The human KCNA2 gene encodes the pore-forming subunit of Kv1.2, a voltage-gated potassium channel. Kv1.2 channels expressed in neurons are therapeutic targets in multiple sclerosis.
Target Gene	KCNA2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNA2
Alias	KCNA2, HK4, Kv1.2, MK2, HBK5, NGK1, RBK2, HUKIV, KV1.2, MGC50217
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNA2 potassium voltage-gated channel, shaker-related subfamily, member 2 [ Homo sapiens ]
Gene Symbol	KCNA2
Synonyms	KCNA2; potassium voltage-gated channel, shaker-related subfamily, member 2; potassium voltage-gated channel subfamily A member 2; HK4; Kv1.2; voltage-gated K(+) channel HuKIV; voltage-gated potassium channel HBK5; voltage-gated potassium channel protein Kv1.2; voltage-gated potassium channel subunit Kv1.2; MK2; HBK5; NGK1; RBK2; HUKIV; KV1.2; MGC50217;
GeneID	3737
Uni ProtID	P16389
mRNA Refseq	BC043564
Chromosome Location	1p13
Function	delayed rectifier potassium channel activity; ion channel activity; potassium channel activity; voltage-gated ion channel activity; voltage-gated potassium channel activity; voltage-gated potassium channel activity;
Pathway	Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	176262

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