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Human HRH1 Stable Cell Line-CHO

Human HRH1 Stable Cell Line-CHO

Cat.No. :  CSC-RG0864 Host Cell:  CHO-K1

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Cell Line Information

Cell Culture Information

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Cat. No. CSC-RG0864
Background Histamine is a ubiquitous messenger that mediates a large number of physiological processes. Its actions are mediated by four receptors named H1, H2, H3 and H4 receptors. The H1 histamine receptor is expressed primarily in lung, vasculature and brain where it mediates smooth muscle contraction, food intake and sleep, amongst other actions. Perhaps most notably, antagonists to this receptor are frequently prescribed to treat respiratory allergies.
Gene HRH1
Gene Species Homo sapiens (Human)
Alias HRH1, H1R, HH1R, H1-R, hisH1
Host Cell CHO-K1
Species Cricetulus griseus (Chinese hamster)
Morphology Epithelial-like
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Research on the mechanisms of GPCR-related diseases

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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