Cat.No. : CSC-RG0062 Host Cell: CHO-K1
| Cat. No. | CSC-RG0062 |
| Background | The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM). |
| Gene | GCGR |
| Gene Species | Homo sapiens (Human) |
| Alias | GCGR, GGR, GL-R, FLJ97182, MGC138246 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human GCGR Stable Cell Line-CHO offers several unique advantages in drug screening, including its high ability to mimic human physiological conditions, which makes the screening results more reflective of human responses. Additionally, the CHO cell line is known for its high expression capabilities and stability, which helps to improve the efficiency and accuracy of the screening process. This cell line also has low batch-to-batch variability, which is crucial for ensuring consistent experimental results.
A: The Human GCGR Stable Cell Line-CHO plays a crucial role in the development of diabetes treatment drugs. As this cell line stably expresses the human glucocorticoid receptor, it can be used to simulate how drugs affect insulin secretion and blood glucose regulation. This helps in screening potential anti-diabetic drugs, assessing their impact on blood glucose levels, and studying the safety and efficacy of the drugs.
A: Optimizing gene expression levels in the Human GCGR Stable Cell Line-CHO typically involves adjusting the components of the culture medium, using specific inducers, and optimizing cell culture conditions. For example, altering the ratios of amino acids, vitamins, and growth factors in the culture medium can enhance expression efficiency. Additionally, the use of chemical or biological inducers can further enhance gene expression. Regular monitoring of cell growth and expression levels, as well as appropriate cell passaging, are also key to maintaining and improving gene expression.
A: The applications of the Human GCGR Stable Cell Line-CHO in studying hormone signaling pathways include analyzing how hormones activate downstream signaling molecules through the GCGR receptor and how these signals affect cell functions. This cell line can be used to study the molecular mechanisms of hormone signaling, such as gene transcription regulation, cell proliferation, and apoptosis. Additionally, it can be used to screen and identify small molecule compounds that affect hormone signaling pathways, providing a foundation for the development of new hormone therapeutic drugs.
A: The role of the Human GCGR Stable Cell Line-CHO in drug metabolism research is primarily to simulate the drug metabolism process in the human liver. Since CHO cell lines typically have low metabolic activity, by stably expressing the human GCGR in these cells, one can study how drugs are metabolized in the human body and the effects of metabolites on cell function. This is significant for predicting the pharmacokinetic properties and potential toxic effects of drugs.
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