Human ERG Stable Cell Line-HEK293

Name: Human ERG Stable Cell Line-HEK293
SKU: CSC-RI0022
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0022

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0022
Description	This cell line is engineered to overexpress human ERG.
Background	The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr.
Target Gene	ERG
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuERG
Alias	ERG, erg 3, p55, erg-3
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ERG v-ets erythroblastosis virus E26 oncogene homolog (avian) [ Homo sapiens ]
Gene Symbol	ERG
Synonyms	p55; erg-3
Gene Description	v-ets erythroblastosis virus E26 oncogene homolog (avian)
GeneID	2078
Uni ProtID	B4DN83
mRNA Refseq	NM_001136154.1
Protein Refseq	NP_001129626.1
Chromosome Location	21q22.3
Function	DNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; signal transducer activity;
Pathway	Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
MIM	165080

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Q & A

Customer Reviews

Customer Q&As

How was the Human ERG Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human ERG (ether-à-go-go related gene) gene into HEK293 cells.

What are the main applications of the Human ERG Stable Cell Line-HEK293 in cardiology research?

A: It is primarily used to study the role of ERG in cardiac electrophysiology and arrhythmias.

What are the advantages of using the Human ERG Stable Cell Line-HEK293 for drug screening?

A: It is suitable for screening drugs affecting ERG channel activity, especially in developing new antiarrhythmic medications.

What should be noted when conducting cell signaling research with the Human ERG Stable Cell Line-HEK293?

A: Special attention should be paid to the impact of drugs or chemicals on ERG channel function and monitoring the electrophysiological changes in cells.

What are the potential uses of the Human ERG Stable Cell Line-HEK293 in cardiac disease model research?

A: It can be used to establish cellular models of arrhythmias and other cardiac diseases, studying the molecular mechanisms of these conditions.

What aspects need special attention when maintaining the Human ERG Stable Cell Line-HEK293?

A: Regular testing for cell division and gene expression should be conducted to ensure stable expression of the ERG gene and consistency in cell characteristics.

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