Cat.No. : CSC-RI0022 Host Cell: HEK293
| Cat. No. | CSC-RI0022 |
| Description | This cell line is engineered to overexpress human ERG. |
| Background | The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr. |
| Gene | ERG |
| Gene Species | Homo sapiens (Human) |
| Alias | ERG, erg 3, p55, erg-3 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line was established by stably transfecting the human ERG (ether-à-go-go related gene) gene into HEK293 cells.
A: It is primarily used to study the role of ERG in cardiac electrophysiology and arrhythmias.
A: It is suitable for screening drugs affecting ERG channel activity, especially in developing new antiarrhythmic medications.
A: Special attention should be paid to the impact of drugs or chemicals on ERG channel function and monitoring the electrophysiological changes in cells.
A: It can be used to establish cellular models of arrhythmias and other cardiac diseases, studying the molecular mechanisms of these conditions.
A: Regular testing for cell division and gene expression should be conducted to ensure stable expression of the ERG gene and consistency in cell characteristics.
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