Cat.No. : CSC-RI0021 Host Cell: CHO-K1
| Cat. No. | CSC-RI0021 |
| Description | This cell line is engineered to overexpress human ERG. |
| Background | The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr. |
| Gene | ERG |
| Gene Species | Homo sapiens (Human) |
| Alias | ERG, erg 3, p55, erg-3 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line is used to evaluate the effects of drugs on cardiac repolarization, particularly the inhibitory effects on hERG channels (key factors affecting the cardiac delayed rectifier potassium current IKr), to assess the potential risk of arrhythmia.
A: Utilize automated cell analysis systems and detection methods suitable for high-throughput screening, such as fluorescence-based assays, to improve the efficiency and accuracy of screening.
A: Use precise electrophysiological detection equipment, such as automated patch clamp techniques, ensuring standardized experimental operations and accurate data collection.
A: Use antibodies or fluorescent probes specific to the ERG channel, combined with techniques like real-time PCR or Western Blot, to ensure the specificity and sensitivity of the detection signals.
A: Perform cross-validation with other cell models or in vitro cardiac models, and use multi-parameter analysis methods to evaluate the comprehensive effects of drugs, thereby reducing false positives.
A: Regularly perform genetic stability tests, such as karyotyping or gene sequencing, and ensure consistent culture conditions, avoiding over-passaging to maintain phenotypic stability.
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