Human ERG Stable Cell Line-CHO-K1

Name: Human ERG Stable Cell Line-CHO-K1
SKU: CSC-RI0021
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0021

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0021
Description	This cell line is engineered to overexpress human ERG.
Background	The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr.
Target Gene	ERG
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuERG
Alias	ERG, erg 3, p55, erg-3
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ERG v-ets erythroblastosis virus E26 oncogene homolog (avian) [ Homo sapiens ]
Gene Symbol	ERG
Synonyms	p55; erg-3
Gene Description	v-ets erythroblastosis virus E26 oncogene homolog (avian)
GeneID	2078
Uni ProtID	B4DN83
mRNA Refseq	NM_001136154.1
Protein Refseq	NP_001129626.1
Chromosome Location	21q22.3
Function	DNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; signal transducer activity;
Pathway	Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
MIM	165080

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Q & A

Customer Reviews

Customer Q&As

What is the application of Human ERG Stable Cell Line-CHO in drug cardiotoxicity assessment?

A: This cell line is used to evaluate the effects of drugs on cardiac repolarization, particularly the inhibitory effects on hERG channels (key factors affecting the cardiac delayed rectifier potassium current IKr), to assess the potential risk of arrhythmia.

How to ensure the efficiency of high-throughput screening using Human ERG Stable Cell Line-CHO in drug screening?

A: Utilize automated cell analysis systems and detection methods suitable for high-throughput screening, such as fluorescence-based assays, to improve the efficiency and accuracy of screening.

How to improve the reliability of data in electrophysiological studies using Human ERG Stable Cell Line-CHO?

A: Use precise electrophysiological detection equipment, such as automated patch clamp techniques, ensuring standardized experimental operations and accurate data collection.

How to enhance the detection of specific signals in cell signaling studies using Human ERG Stable Cell Line-CHO?

A: Use antibodies or fluorescent probes specific to the ERG channel, combined with techniques like real-time PCR or Western Blot, to ensure the specificity and sensitivity of the detection signals.

How to reduce false-positive results in drug safety assessment using Human ERG Stable Cell Line-CHO?

A: Perform cross-validation with other cell models or in vitro cardiac models, and use multi-parameter analysis methods to evaluate the comprehensive effects of drugs, thereby reducing false positives.

How to maintain the genetic stability and phenotypic consistency of Human ERG Stable Cell Line-CHO during long-term culture?

A: Regularly perform genetic stability tests, such as karyotyping or gene sequencing, and ensure consistent culture conditions, avoiding over-passaging to maintain phenotypic stability.

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