Cat.No. : CSC-RG0003 Host Cell: HEK293
| Cat. No. | CSC-RG0003 |
| Background | CNR1 gene encodes a protein that is one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the family of guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dosedependent, stereoselective and pertussis toxinsensitive manner. The two receptors have been found to be involved in the cannabinoidinduced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Two transcript variants encoding different isoforms have been described for this gene. |
| Gene | CNR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CNR1, CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CNR1 Stable Cell Line-HEK293, which expresses the CB1 receptor, provides a platform for studying the pharmacological properties of this receptor. Researchers can investigate the binding affinity, efficacy, and selectivity of various ligands, including endocannabinoids and synthetic cannabinoids, to the CB1 receptor. This cell line is instrumental in understanding the molecular mechanisms of cannabinoid action and can contribute to the development of new drugs targeting the endocannabinoid system.
A: The Human CNR1 Stable Cell Line-HEK293 is a valuable tool for studying the role of the CB1 receptor in neurological processes. By examining the effects of cannabinoids on this receptor, researchers can gain insights into potential therapeutic applications for conditions such as epilepsy, multiple sclerosis, and Parkinson's disease. The cell line can be used to screen compounds for their efficacy and safety, helping to advance the development of cannabinoid-based therapies.
A: The Human CNR1 Stable Cell Line-HEK293 allows for the investigation of receptor desensitization and downregulation in response to chronic cannabinoid exposure. This is crucial for understanding the long-term effects of cannabinoid use and the development of tolerance, which are significant considerations in the context of medical marijuana and recreational cannabis use.
A: The Human CNR1 Stable Cell Line-HEK293 is a useful model for studying the CB1 receptor's role in various disorders associated with the endocannabinoid system. By examining the effects of potential therapeutic compounds on the CB1 receptor, researchers can identify new drug candidates and optimize their efficacy and safety profiles. This cell line can contribute significantly to the development of drugs for conditions such as chronic pain, epilepsy, and addiction.
A: The Human CNR1 Stable Cell Line-HEK293 can be used to study the CB1 receptor's role in appetite regulation, as this receptor is known to be involved in the central regulation of food intake. By examining the effects of cannabinoids on the CB1 receptor in this cell line, researchers can gain insights into the molecular mechanisms that underlie the orexigenic (appetite-stimulating) effects of cannabinoids, which could inform the development of treatments for obesity and related disorders.
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