Human CHRNA7 Stable Cell Line-HEK293

Name: Human CHRNA7 Stable Cell Line-HEK293
SKU: CSC-RI0020
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0020

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0020
Description	This cell line is engineered to overexpress human CHRNA7.
Target Gene	CHRNA7
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCHRNA7
Alias	CHRNA7, NACHRA7, CHRNA7-2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRNA7 cholinergic receptor, nicotinic, alpha 7 (neuronal) [ Homo sapiens ]
Gene Symbol	CHRNA7
Synonyms	CHRNA7; cholinergic receptor, nicotinic, alpha 7 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylcholine receptor subunit alpha-7; acetylcholine receptor; nicotinic; alpha 7 (neuronal); a7 nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor; acetylcholine receptor, nicotinic, alpha 7 (neuronal); neuronal acetylcholine receptor protein, alpha-7 chain; NACHRA7; CHRNA7-2;
GeneID	1139
Uni ProtID	P36544
mRNA Refseq	BC037571
Chromosome Location	15q13.3
Function	acetylcholine binding; acetylcholine receptor activity; acetylcholine-activated cation-selective channel activity; beta-amyloid binding; chloride channel regulator activity; extracellular ligand-gated ion channel activity; ion channel activity; protein homodimerization activity; receptor activity; toxin binding;
Pathway	Acetylcholine Binding And Downstream Events, organism-specific biosystem; Activation of Nicotinic Acetylcholine Receptors, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
MIM	118511

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alpha7 nicotinic acetylcholine (nACh7) receptors are predominantly expressed in the CNS, including the hippocampus, cortex and ventral tegmental area. Nicotinic ACh7 receptors are involved with LTP, memory and attention deficits and so are drug development targets for diseases of cognition including Alzheimer's and schizophrenia. The nACh7 receptors are rapidly desensitized, have homopentameric architecture and respond to agonists including nicotine, acetylcholine, AR-R17779 and epibatidine. In addition the currents are blocked by the competitive antagonist methyllycaconitine. Drug discovery efforts have also identified positive allosteric modulators such as PNU-120596 which increase the potency of activators such as acetylcholine without causing desensitization. This cell line also expresses the ric3 chaperone protein as when co-expressed with the nACh7 receptor, ric-3 significantly increases surface expression.

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