Human HTR3A Stable Cell Line-HEK293

Name: Human HTR3A Stable Cell Line-HEK293
SKU: CSC-RI0059
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0059

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0059
Description	This cell line is engineered to overexpress human HTR3A.
Background	The human HTR3A gene encodes the pore-forming subunit of 5-HT3A, a ligand-gated cation channel activated by 5-hydroxytryptamine (serotonin). 5-HT3A channels expressed in the CNS and GI tract, are therapeutic targets in nausea and irritable bowel syndrome.
Target Gene	HTR3A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuHTR3A
Alias	HTR3A, 5 HT3A, 5 HT3R, 5-HT3-A, HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HTR3A 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic [ Homo sapiens ]
Gene Symbol	HTR3A
Synonyms	HTR3A; 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic; 5 hydroxytryptamine (serotonin) receptor 3A , HTR3; 5-hydroxytryptamine receptor 3A; 5 HT3A; 5 HT3R; 5-HT3-A; serotonin receptor 3A; 5HT3 serotonin receptor; 5-hydroxytryptamine receptor 3; serotonin-gated ion channel receptor; HTR3; 5HT3R; 5-HT-3; 5-HT3A; 5-HT3R;
GeneID	3359
Uni ProtID	P46098
mRNA Refseq	BC002354
Chromosome Location	11q23.1-q23.2
Function	extracellular ligand-gated ion channel activity; extracellular ligand-gated ion channel activity; ion channel activity; receptor activity; serotonin binding; serotonin receptor activity; serotonin receptor activity; serotonin-activated cation-selective channel activity;
Pathway	Ion channel transport, organism-specific biosystem; Ligand-gated ion channel transport, organism-specific biosystem; Serotonergic synapse, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem;
MIM	182139

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Customer Reviews

Customer Q&As

What special applications does this cell line have in neuroscience research?

A: It is primarily used for studying serotonin signaling in the nervous system, especially its role in mood regulation and anxiety disorders.

What are the potential uses of the Human HTR3A Stable Cell Line-HEK293 in gastrointestinal disease research?

A: Since serotonin receptors play a significant role in gastrointestinal function, this cell line can be used to study signaling pathways related to gastrointestinal disorders.

How to ensure the reproducibility of experiments using the Human HTR3A Stable Cell Line-HEK293?

A: Ensure consistency in experimental conditions, including cell density, composition of the culture medium, and experimental procedures.

What are the advantages of using the Human HTR3A Stable Cell Line-HEK293 in drug development?

A: It can be used for high-throughput screening of drugs affecting serotonin receptor activity, particularly antidepressants and anti-anxiety medications.

What are the applications of the Human HTR3A Stable Cell Line-HEK293 in pharmacological toxicity assessment?

A: It can be used to assess the potential toxicity of drugs on serotonin receptors, especially their impact on the central nervous system.

How was the Human HTR3A Stable Cell Line-HEK293 constructed, and what gene engineering technique was used?

A: The cell line was constructed by stably transfecting the human HTR3A (5-hydroxytryptamine receptor 3A) gene into HEK293 cells, using standard gene transfection and selection techniques.

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