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Cat.No. : CSC-RI0059 Host Cell: HEK293
| Cat. No. | CSC-RI0059 |
| Description | This cell line is engineered to overexpress human HTR3A. |
| Background | The human HTR3A gene encodes the pore-forming subunit of 5-HT3A, a ligand-gated cation channel activated by 5-hydroxytryptamine (serotonin). 5-HT3A channels expressed in the CNS and GI tract, are therapeutic targets in nausea and irritable bowel syndrome. |
| Gene | HTR3A |
| Gene Species | Homo sapiens (Human) |
| Alias | HTR3A, 5 HT3A, 5 HT3R, 5-HT3-A, HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: It is primarily used for studying serotonin signaling in the nervous system, especially its role in mood regulation and anxiety disorders.
A: Since serotonin receptors play a significant role in gastrointestinal function, this cell line can be used to study signaling pathways related to gastrointestinal disorders.
A: Ensure consistency in experimental conditions, including cell density, composition of the culture medium, and experimental procedures.
A: It can be used for high-throughput screening of drugs affecting serotonin receptor activity, particularly antidepressants and anti-anxiety medications.
A: It can be used to assess the potential toxicity of drugs on serotonin receptors, especially their impact on the central nervous system.
A: The cell line was constructed by stably transfecting the human HTR3A (5-hydroxytryptamine receptor 3A) gene into HEK293 cells, using standard gene transfection and selection techniques.
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