Human CD27 Stable Cell Line - GS-H2

Name: Human CD27 Stable Cell Line - GS-H2
SKU: CSC-RO0229
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0229

Host Cell : GS-H2 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0229
Description	This cell line is engineered to stably overexpress the human CD27 in GS-H2 cells.
Introduction	GS-H2/CD27 cell line stably expresses human CD27 molecule. The expression of CD27 in this cell line has been validated through flow cytometry. IL-8 production can be detected in this cell line when it is treated with CD70.
Target Gene	CD27
Gene Species	Homo sapiens (Human)
Host Cell	GS-H2
Source	GS-H2
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD27 CD27 molecule [ Homo sapiens ]
Gene Symbol	CD27
Synonyms	T14; S152; Tp55; TNFRSF7
Gene Description	CD27 molecule
Gene ID	939
Uni Prot ID	P26842
m RNA Refseq	NM_001242.4
Protein Refseq	NP_001233.1
Chromosome Location	12p13
Function	cysteine-type endopeptidase inhibitor activity involved in apoptotic process; protein binding; transmembrane signaling receptor activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
MIM	186711

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