Human CD27 Stable Cell Line-CHO-K1

Name: Human CD27 Stable Cell Line-CHO-K1
SKU: CSC-RO0066
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0066

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0066
Description	This cell line is a stably transfected cell line which expresses human CD27 molecule without any tag.
Background	CD27 protein is a member of the TNF-receptor superfamily. It is required for generation and long-term maintenance of T cell immunity. This receptor binds to ligand CD70, and plays a crytical role in regulating B-cell activation and immunoglobulin synthesis. It transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Target	Human
Target Gene	CD27
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD27 CD27 molecule [ Homo sapiens (human) ]
Gene Symbol	CD27
Synonyms	T14; S152; Tp55; TNFRSF7; S152. LPFS2
Gene ID	939
Uni Prot ID	P26842
Chromosome Location	12p13.31

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is CD27?

A: CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes T cell co-stimulatory receptors OX40, 4-1BB, and Herpes Virus Entry Mediator (HVEM).

On which cells is CD27 expressed?

A: CD27 is expressed on various types of T cells, B cell subgroups, and natural killer cells.

How does CD27 function?

A: CD27 activates NF-kB and MAPK/JNK signaling through its interaction with its TNF-like ligand CD70, whose expression is driven by many tumor cells.

What is the effect of activating CD27?

A: Activation of CD27 leads to lymphocyte proliferation, differentiation, cell apoptosis, and induction of long-term memory. The CD27/CD70 pathway is being developed for the treatment of cancer and inflammatory diseases.

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