Cat.No. : CSC-RO0066 Host Cell: CHO-K1
| Cat. No. | CSC-RO0066 |
| Description | This cell line is a stably transfected cell line which expresses human CD27 molecule without any tag. |
| Background | CD27 protein is a member of the TNF-receptor superfamily. It is required for generation and long-term maintenance of T cell immunity. This receptor binds to ligand CD70, and plays a crytical role in regulating B-cell activation and immunoglobulin synthesis. It transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| Gene | CD27 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Target | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CD27 CD27 molecule [ Homo sapiens (human) ] |
| Gene Symbol | CD27 |
| Synonyms | T14; S152; Tp55; TNFRSF7; S152. LPFS2 |
| Gene ID | 939 |
| UniProt ID | P26842 |
| Chromosome Location | 12p13.31 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes T cell co-stimulatory receptors OX40, 4-1BB, and Herpes Virus Entry Mediator (HVEM).
A: CD27 is expressed on various types of T cells, B cell subgroups, and natural killer cells.
A: CD27 activates NF-kB and MAPK/JNK signaling through its interaction with its TNF-like ligand CD70, whose expression is driven by many tumor cells.
A: Activation of CD27 leads to lymphocyte proliferation, differentiation, cell apoptosis, and induction of long-term memory. The CD27/CD70 pathway is being developed for the treatment of cancer and inflammatory diseases.
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