Cat.No. : CSC-RG0041 Host Cell: HEK293
| Cat. No. | CSC-RG0041 |
| Gene | CCR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CCR1, CMKBR1, SCYAR1, CKR 1, MIP1aR, CCR-1, CKR1, CKR-1, HM145, CMKBR1, SCYAR1 |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: By setting up a chemical gradient in the Human CCR1-SNAP Stable Cell Line-HEK293, one can observe how chemokines influence cell migration behavior. Tracking the dynamic changes of the CCR1 receptor with the SNAP tag under a fluorescence microscope allows for the study of cytoskeletal rearrangements and cell polarization following receptor activation.
A: This cell line enables researchers to directly observe the activation state of the CCR1 receptor through the SNAP tag, which is crucial for real-time monitoring of signaling events. Additionally, the flexibility of the SNAP tag allows for various biochemical and biophysical experiments, such as FRET and co-immunoprecipitation, to gain a deeper understanding of the signaling mechanisms of the CCR1 receptor.
A: The endocytosis of the CCR1 receptor can be assessed by using endocytosis inhibitors and tracking the endocytosis process of the SNAP-tagged receptor. Time-resolved fluorescence imaging allows for the observation of how the CCR1 receptor is internalized and enters the endoplasmic reticulum after activation.
A: This cell line provides a platform for studying how the CCR1 receptor couples with G proteins and activates downstream signaling pathways. Monitoring the activation and dissociation of G proteins through the SNAP tag is crucial for understanding the signaling mechanisms of the CCR1 receptor.
A: The expression levels of the CCR1 receptor can be quantitatively analyzed using Western Blot or flow cytometry. Western Blot provides information about the total amount of CCR1 receptor protein, while flow cytometry assesses the expression levels of the CCR1 receptor in individual cells.
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