Human CCR1-SNAP Stable Cell Line-HEK293

Name: Human CCR1-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0041
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0041

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0041
Target Gene	CCR1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCCR1-SNAP
Alias	CCR1, CMKBR1, SCYAR1, CKR 1, MIP1aR, CCR-1, CKR1, CKR-1, HM145, CMKBR1, SCYAR1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CCR1 chemokine (C-C motif) receptor 1 [ Homo sapiens ]
Gene Symbol	CCR1
Synonyms	CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
Gene Description	chemokine (C-C motif) receptor 1
Gene ID	1230
Uni Prot ID	P32246
m RNA Refseq	NM_001295.2
Protein Refseq	NP_001286.1
Chromosome Location	3p21
Function	C-C chemokine binding; C-C chemokine receptor activity; chemokine (C-C motif) ligand 5 binding; chemokine (C-C motif) ligand 7 binding; chemokine receptor activity; phosphatidylinositol phospholipase C activity; protein binding;
Pathway	Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem;
MIM	601159

Quick Inquiry

Background

Q & A

Customer Reviews

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p.

Customer Q&As

How can the Human CCR1-SNAP Stable Cell Line-HEK293 be used to study the effects of chemokines on cell migration?

A: By setting up a chemical gradient in the Human CCR1-SNAP Stable Cell Line-HEK293, one can observe how chemokines influence cell migration behavior. Tracking the dynamic changes of the CCR1 receptor with the SNAP tag under a fluorescence microscope allows for the study of cytoskeletal rearrangements and cell polarization following receptor activation.

What are the unique advantages of the Human CCR1-SNAP Stable Cell Line-HEK293 in studying CCR1 receptor signaling?

A: This cell line enables researchers to directly observe the activation state of the CCR1 receptor through the SNAP tag, which is crucial for real-time monitoring of signaling events. Additionally, the flexibility of the SNAP tag allows for various biochemical and biophysical experiments, such as FRET and co-immunoprecipitation, to gain a deeper understanding of the signaling mechanisms of the CCR1 receptor.

How can the endocytosis of the CCR1 receptor be assessed in the Human CCR1-SNAP Stable Cell Line-HEK293?

A: The endocytosis of the CCR1 receptor can be assessed by using endocytosis inhibitors and tracking the endocytosis process of the SNAP-tagged receptor. Time-resolved fluorescence imaging allows for the observation of how the CCR1 receptor is internalized and enters the endoplasmic reticulum after activation.

What role does the Human CCR1-SNAP Stable Cell Line-HEK293 play in studying the mechanism of CCR1 receptor coupling with G proteins?

A: This cell line provides a platform for studying how the CCR1 receptor couples with G proteins and activates downstream signaling pathways. Monitoring the activation and dissociation of G proteins through the SNAP tag is crucial for understanding the signaling mechanisms of the CCR1 receptor.

How can the expression levels of the CCR1 receptor be quantitatively analyzed in the Human CCR1-SNAP Stable Cell Line-HEK293?

A: The expression levels of the CCR1 receptor can be quantitatively analyzed using Western Blot or flow cytometry. Western Blot provides information about the total amount of CCR1 receptor protein, while flow cytometry assesses the expression levels of the CCR1 receptor in individual cells.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *