Human CCKBR-SNAP Stable Cell Line-HEK293

Name: Human CCKBR-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0046
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0046

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0046
Background	This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.
Target Gene	CCKBR
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCCKBR-SNAP
Alias	CCKBR, CCK-BR, CCK2-R, GASR, CCK-B, CCK2R
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CCKBR cholecystokinin B receptor [ Homo sapiens ]
Gene Symbol	CCKBR
Synonyms	CCKBR; cholecystokinin B receptor; gastrin/cholecystokinin type B receptor; CCK-BR; CCK2-R; CCK2 receptor; CCK-B receptor; gastrin receptor; cholecystokinin-2 receptor; GASR; CCK-B; CCK2R;
Gene ID	887
Uni Prot ID	P32239
m RNA Refseq	BC000740
Chromosome Location	11p15.4
Function	1-phosphatidylinositol-3-kinase regulator activity; G-protein coupled receptor activity; cholecystokinin receptor activity; gastrin receptor activity; phosphatidylinositol phospholipase C activity; receptor activity; signal transducer activity; type B gastrin/cholecystokinin receptor binding;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem;
MIM	118445

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Customer Q&As

How does the Human CCKBR-SNAP Stable Cell Line-HEK293 enhance the study of CCKBR (Cholecystokinin B receptor) signaling in neuropsychiatric disorders?

A: The Human CCKBR-SNAP Stable Cell Line-HEK293 provides a platform for investigating the role of CCKBR in neuropsychiatric disorders by allowing researchers to study the receptor's function in a controlled environment. The SNAP tag enables the visualization and tracking of CCKBR activity, which can help identify potential therapeutic targets for conditions such as anxiety, depression, and addiction, where CCKBR has been implicated.

How does the Human CCKBR-SNAP Stable Cell Line-HEK293 contribute to the development of novel imaging agents for CCKBR?

A: The SNAP tag in this cell line allows for the attachment of fluorescent or luminescent probes, which can be used to develop imaging agents that target CCKBR. These agents could be used for in vivo imaging studies to visualize the distribution and activity of CCKBR in the brain, aiding in the understanding of its role in various physiological processes and diseases.

How does the Human CCKBR-SNAP Stable Cell Line-HEK293 facilitate the study of CCKBR in the context of cancer biology?

A: The cell line can be utilized to investigate the role of CCKBR in cancer cell proliferation, migration, and survival. By studying the receptor's function in cancerous HEK293 cells, researchers can gain insights into potential cancer therapeutic strategies that involve targeting CCKBR.

What are the ethical implications of using the Human CCKBR-SNAP Stable Cell Line-HEK293 for research purposes?

A: While cell lines like HEK293 are widely used in research, ethical considerations include ensuring the cells are obtained and used responsibly, maintaining transparency about their origin, and adhering to guidelines for cell line authentication and quality control to prevent misidentification and contamination.

How does the Human CCKBR-SNAP Stable Cell Line-HEK293 support the development of new therapeutic strategies for CCKBR-related disorders?

A: By providing a model system to study CCKBR's function and modulation, this cell line can help researchers identify novel therapeutic targets and test the efficacy of potential drugs. The ability to model receptor activity in a controlled environment allows for the development of strategies that could lead to more effective treatments for disorders associated with CCKBR dysregulation.

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