Cat.No. : CSC-RG0046 Host Cell: HEK293
| Cat. No. | CSC-RG0046 |
| Background | This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. |
| Gene | CCKBR |
| Gene Species | Homo sapiens (Human) |
| Alias | CCKBR, CCK-BR, CCK2-R, GASR, CCK-B, CCK2R |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CCKBR-SNAP Stable Cell Line-HEK293 provides a platform for investigating the role of CCKBR in neuropsychiatric disorders by allowing researchers to study the receptor's function in a controlled environment. The SNAP tag enables the visualization and tracking of CCKBR activity, which can help identify potential therapeutic targets for conditions such as anxiety, depression, and addiction, where CCKBR has been implicated.
A: The SNAP tag in this cell line allows for the attachment of fluorescent or luminescent probes, which can be used to develop imaging agents that target CCKBR. These agents could be used for in vivo imaging studies to visualize the distribution and activity of CCKBR in the brain, aiding in the understanding of its role in various physiological processes and diseases.
A: The cell line can be utilized to investigate the role of CCKBR in cancer cell proliferation, migration, and survival. By studying the receptor's function in cancerous HEK293 cells, researchers can gain insights into potential cancer therapeutic strategies that involve targeting CCKBR.
A: While cell lines like HEK293 are widely used in research, ethical considerations include ensuring the cells are obtained and used responsibly, maintaining transparency about their origin, and adhering to guidelines for cell line authentication and quality control to prevent misidentification and contamination.
A: By providing a model system to study CCKBR's function and modulation, this cell line can help researchers identify novel therapeutic targets and test the efficacy of potential drugs. The ability to model receptor activity in a controlled environment allows for the development of strategies that could lead to more effective treatments for disorders associated with CCKBR dysregulation.
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