Human CCKBR Stable Cell Line - HEK293

Name: Human CCKBR Stable Cell Line - HEK293
SKU: CSC-RO01386

Cat.No. : CSC-RO01386 Host Cell: Human immortalized embryonic kidney cell line (HEK293)

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Gene Informationn

Cat. No.	CSC-RO01386
Description	This cell line is engineered to stably express Homo sapiens (human) cholecystokinin B receptor (CCKBR) in Human immortalized embryonic kidney cell line (HEK293). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Reporter	GFP
Gene	CCKBR
Gene Species	Homo sapiens (human)
Host Cell	Human immortalized embryonic kidney cell line (HEK293)
Host Cell Species	Homo sapiens (human) cell line
Stability	This cell line is stable at least 10 passages.
Product Type	Human gene overexpression stable cell line
Applications	1) investigation of gene function 2) screening and validation of antibodies
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Gene Name	Cckbr cholecystokinin B receptor [ Mus musculus ]
Gene Symbol	Cckbr
Synonyms	CCK2R; CCKR-2
Gene Description	cholecystokinin B receptor
Gene ID	12426
UniProt ID	P56481
mRNA Refseq	NM_007627.4
Protein Refseq	NP_031653.1
Chromosome Location	7 E3; 7 50.0 cM
Function	G-protein coupled receptor activity; gastrin receptor activity; protein binding; signal transducer activity; type B gastrin/cholecystokinin receptor binding;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem;

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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The CCKBR gene encodes the cholecystokinin B receptor, a G protein-coupled receptor (GPCR) with high affinity for both gastrin and cholecystokinin. CCKBR is primarily expressed in the central nervous system and gastric mucosa, stimulating gastric acid secretion. Clinically, CCKBR is frequently overexpressed in various malignancies, including small cell lung cancer and medullary thyroid carcinoma. Its overactivation can promote tumor cell survival by activating the MAPK/ERK and PI3K/Akt signaling pathways. CCKBR is also currently being investigated as a target for radionuclide therapy of neuroendocrine tumors.

A stable human CCKBR cell line in the HEK293 context is an ideal model for studying the molecular pharmacology of the gastrin receptor. HEK293 cells provide a standardized environment for studying GPCR signaling and receptor-ligand interactions. By providing a stable platform for human CCKBR expression, this cell line allows researchers to precisely quantify the binding kinetics of various peptide ligands and evaluate the functional activity of novel antagonists. This product is of invaluable value for high-throughput screening of CCKBR modulators and for validating the specificity of next-generation imaging agents.

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